ABSTRACT. Rodrigues A.L., de Souza B.B., Pereira Filho J.M., de Mendonça M.F.F., Marques B.A.A., Silva L. de B., Viturino P.V. & Campos E.M. [Environment influence in first milking upon production, composition, somatic cell count and hormones on cows in system created biodynamic.] Influência do ambiente de pré-ordenha sobre produção, composição, contagem de células somáticas do leite e hormônios de vacas criadas em sistema biodinâmico. Revista Brasileira de Medicina Veterinária, 36(2):174-182, 2014. Programa de Pós- -Graduação em Medicina Veterinária, Centro de Saúde e Tecnologia Rural, Universidade Federal de Campina Grande, Caixa postal 64, Patos, PB 58708- 110, Brasil. E-mail: email@example.com The objective of the present study were apprise in two environments in first milking upon production, composition, SCC of milk and the concentration hormonal plasmatic in cows Brown Swiss cattle in two-production level and system created biodynamic. We used 32 cows; we had 16 in low and 16 in up production. The cow waits 1.5 hour for the first milking on the sun and in without sun. All the cows had the loggers to register the environment variations. The design experimental was completely randomized with 2 x 2 arrangement factorial (two environments first milking and two levels of production), consisted of 4 groups of 8 repetitions each. The acidity (P≤0.01) and the total solids (P>0.05) were less in the milk from the cows put in without sun. The cows about the production less level showed acidity (P≤0.01), density (P>0.05), lactose (P≤0.01) and solids without lipids (P≤0.01) in the milk in cows form milk had low production therefore we did not observation some difference in total solids (P>0.05) between two level production. The SCC milk did not showed variations between the environments and the production level (P>0.05), therefore in the number, were up in the cows with of sun and when had low production. The values form T3, T4 and cortisol, not had variations in the four groups in the study (P>0.05) and was in accord with the literature. When we put the cows Brown Swiss cattle for 1,5 hours in the first milking in system created biodynamic of production showed low influence weather in the cows did not showed good up production and good milk composition.