Brazilian Journal of Veterinary Medicine <p>The Brazilian Journal of Veterinary Medicine was launched in 1979 as the official scientific periodical of the Sociedade de Medicina Veterinária do Estado do Rio de Janeiro (SOMVERJ). </p> <p>The Brazilian Journal of Veterinary Medicine is an open-access journal which means that all content is freely available without charge to the user or their institution. Users are allowed to read, download, copy, distribute, print, search or link to the full texts of the articles, or use them for any other legal purpose, without asking for prior permission from the editor or author, provided they are cited. The font is licensed by Creative Commons Attribution International CC-BY.</p> <p><strong>ISSN 0100-2430</strong></p> <p><strong>ISSN-e 2527-2179.</strong></p> <p> </p> Sociedade de Medicina Veterinária do Estado do Rio de Janeiro. en-US Brazilian Journal of Veterinary Medicine 0100-2430 Environmental enrichment interaction for laboratory beagle dogs used in research <p>Experimental animal facilities can have a negative impact on the well-being of animals owing to confinement. To mitigate this, environmental enrichment (EE) is implemented confinement. The purpose of EE is to enhance the complexity of an animal’s natural environment. The objective of this study was to identify the types of EE most enjoyed by dogs used in experimental research and housed in individual kennels. A total of six adult Beagle dogs, housed at the Laboratório de Quimioterapia Experimental em Parasitologia Veterinária (LQEPV) at the Universidade Federal Rural do Rio de Janeiro (UFRRJ) were included in the study. The EE tools used included Petball® toys, a grassy outdoor area, interaction with other dogs and with a team member, a “pool” made of plastic bottles, and dog’s wet food ice cream. A team member assessed the usage of these tools every 5 min for a 30-min period, six times per day, one day per week, over the course of eight weeks. The study revealed that the grass area was the tool most commonly used for physical enrichment, accounting for 58% of the occurrences (<em>p </em>&lt; 0.05). Social and food enrichment were enjoyed in second and third place, with 23% and 19% of occurrences, respectively. that the study findings suggest that dogs housed in individual kennels enjoy engaging in their natural behaviors.</p> Anna Julia Bessa Fernandes Fernanda da Silva Freitas Campos Gabriella Santos Oliveira Priscila Cardim Oliveira Debora Azevedo Borges Ivan de Alamar Pedrosa Fabio Barbour Scott Copyright (c) 2024 Anna Julia Bessa Fernandes, Fernanda da Silva Freitas Campos, Gabriella Santos Oliveira, Priscila Cardim Oliveira, Debora Azevedo Borges, Ivan de Alamar Pedrosa, Fabio Barbour Scott 2024-02-16 2024-02-16 46 e006323 e006323 10.29374/2527-2179.bjvm006323 Lippia sidoides essential oil at concentration of 0.25% provided improvements in microbiota and intestine integrity of Danio rerio <p>The study evaluated the effects of dietary supplementation with <em>Lippia sidoides </em>essential oil on the microbiota and intestinal morphology of <em>Danio rerio</em>. For this, 448 fish were randomly distributed in 28 tanks divided into a control group fed a commercial diet without supplementation, a group fed a commercial diet containing grain alcohol and five groups fed a commercial diet containing essential oil of <em>L. sidoides </em>(LSEO) at concentrations of 0.25%, 0.50%, 0.75%, 1.00% and 1.25%. After the period of dietary supplementation, biological materials were collected for microbiological and histological analyses. There were no significant differences regarding the microbiological count between the groups. Diversity of the microbiome was higher in 0.25% group than in control group. LSEO inhibited the growth of potentially pathogenic bacteria. Fish fed LSEO0.25% showed greater intestinal histomorphometric indices. The inclusion of LSEO at 0.25% in the diet of <em>D. rerio </em>provided improvements in fish microbiota and intestine integrity.</p> Lucas Cardoso Marco Shizuo Owatari Francisco Célio Maia Chaves Tamiris Henrique Ferreira Domickson Silva Costa William Eduardo Furtado Marília Tedesco Luciana Aparecida Honorato José Luiz Pedreira Mouriño Maurício Laterça Martins Copyright (c) 2024 Lucas Cardoso, Marco Shizuo Owatari, Francisco Célio Maia Chaves, Tamiris Henrique Ferreira, Domickson Silva Costa, William Eduardo Furtado, Marília Tedesco, Luciana Aparecida Honorato, José Luiz Pedreira Mouriño, Maurício Laterça Martins 2024-02-14 2024-02-14 46 e005323 e005323 10.29374/2527-2179.bjvm005323 An evaluation of techniques to diagnose Dioctophyme renale in dogs <p><em>Dioctophyme renale </em>is a nematode with zoonotic potential that affects the kidneys of carnivorous, wild, and domestic mammals. In this study, we sought to evaluate the indirect ELISA method against routine methods used to diagnose dioctophimosis. Hence, 38 dogs parasitized by <em>D. renale</em>, as confirmed by surgery, were selected. The dogs were evaluated by abdominal ultrasound and urinalysis, and their sera were tested by indirect ELISA using <em>D. renale </em>adult secretion and excretion antigen (DES). Five dogs were followed up with serum collections on day 0 (day of surgery) and 30, 60, and 90 days after surgery to evaluate antibody kinetics. Abdominal ultrasound and indirect ELISA successfully diagnosed 37 dogs parasitized by <em>D. renale</em>, while urinalysis diagnosed 29 animals. The positive animals were parasitized with 1–7 parasites; 17 dogs were infected by male and female parasites, 15 only by female parasites, and six were parasitized only by male parasites. When assessing specificity and sensitivity, all techniques showed 100% specificity and 81.6%, 97.4%, and 97.4% sensitivity for urinalysis, ultrasound, and ELISA, respectively (<em>p </em>&lt; 0.001). The five positive dogs that were followed up after surgery showed a progressive decrease in mean absorbances in indirect ELISA (0.644, 0.516, 0.511, and 0.440, respectively). This study demonstrated that the indirect ELISA using the DE antigen could diagnose dioctophimosis regardless of the number, sex, and location of the parasites, with the potential to be used in epidemiological research and implementing immunological and molecular studies, opening new lines of research on <em>D. renale. </em></p> Gabriela de Almeida Capella Josaine Cristina da Silva Rappeti Natalia Berne Pinheiro Soliane Carra Perera Micaele Quintana de Moura Marlete Brum Cleff Caroline Maciel da Costa Adriane Leites Strothmann Guilherme Borges Weege Carolina Silveira Mascarenhas Maria Elisabeth Aires Berne Copyright (c) 2024 Gabriela de Almeida Capella, Josaine Cristina da Silva Rappeti, Natalia Berne Pinheiro, Soliane Carra Perera, Micaele Quintana de Moura, Marlete Brum Cleff, Caroline Maciel da Costa, Adriane Leites Strothmann, Guilherme Borges Weege, Carolina Silveira Mascarenhas, Maria Elisabeth Aires Berne 2024-02-05 2024-02-05 46 e006423 e006423 10.29374/2527-2179.bjvm006423 Bovine herpesvirus meningoencephalitis in the State of Tocantins, Brazil <p>Three outbreaks of herpesvirus meningoencephalitis in cattle have been reported in three municipalities in the northern region of the State of Tocantins, Brazil. In one outbreak, 41 predominantly young bovines were affected, with 2–3 deaths in some cases. The animals showed neurological signs of incoordination, blindness, and recumbency, with death occurring within approximately 4–5 d. At necropsy, hyperemia and leptomeningeal hemorrhages were observed in the brain. Histology revealed more intense lesions in the rostral portions of the brain, mainly affecting the frontoparietal cerebral cortex, with nonsuppurative encephalitis and meningitis, glial nodules, neuronophagia, and eosinophilic intranuclear inclusion bodies in the astrocytes and neurons. This study shows the presence of bovine herpesvirus in Tocantins, probably the highly neurotropic type 5 strain, and emphasizes its importance in the differential diagnosis of bovine neuropathies.</p> Ilgner Aimar Bezerra Pinheiro Bianca Pereira Dias Jardel Martins Ferreira Alessandro José Ferreira dos Santos Sandro Estevan Moron Gilzelle Maria da Luz Silva Liana Bezerra Dias de Lima Fabiano Mendes de Cordova Copyright (c) 2024 Ilgner Aimar Bezerra Pinheiro, Bianca Pereira Dias, Jardel Martins Ferreira, Alessandro José Ferreira dos Santos, Sandro Estevan Moron, Gilzelle Maria da Luz Silva, Liana Bezerra Dias de Lima, Fabiano Mendes de Cordova 2024-01-30 2024-01-30 46 E004023 E004023 10.29374/2527-2179.bjvm004023 Use of RNA-seq to identify genes encoding cytokines and chemokines activated following uptake and processing a candidate peptide vaccine developed against Mycobacterium avium subsp. paratuberculosis <p>Analysis of the primary and recall responses to a membrane molecule (MMP), encoded by MAP2121c demonstrated that tri-directional signaling between the antigen-presenting cell (APC), CD4 and CD8 is essential for eliciting a CD8 cytotoxic T cell (CTL) response against Mycobacterium avium subsp. paratuberculosis. As reported here, RNA-sequencing was used to initiate the characterization of the signaling pathways involved in eliciting the development of CD8 CTL, starting with the characterization of the activation status of genes in monocyte-derived macrophages (MoMΦ) following uptake and processing MMP for the presentation of antigenic epitopes to CD4 and CD8 T cells. Activation status was compared with the uptake and processing of LPS, a nonspecific stimulator of macrophages. 1609 genes were identified that were upregulated, and 1277 were downregulated three hours after uptake and processing MMP. No significant difference was observed in the cytokine genes selected for analysis of the signaling that must occur between APC, CD4, and CD8 for the development of CTL. The initial observations indicate screening of the transcriptome should include genes involved in signaling between APC and CD4, and CD8 regardless of their activation status. Four genes of interest in this study, IL12A, IL12B, IL15, and IL23A, were not significantly different from control values. The initial studies also indicate MoMΦ can be included with dendritic cells and monocyte-derived dendritic cells for further analysis of the tri-directional signaling required for the development of CTL.</p> Michelle Athena Decourcey William Charles Davis Cleverson de Souza Copyright (c) 2024 Michelle Athena Decourcey, William Charles Davis, Cleverson de Souza 2024-01-16 2024-01-16 46 e002723 e002723 10.29374/2527-2179.bjvm002723