Effects of fluazuron on the biological parameters of engorged females of Rhipicephalus microplus


growth regulator

How to Cite

Gaudêncio, F. N., Cordeiro, M. D., Rodrigues, J. de A. V., Baêta, B. A., Bittencourt, V. R. E. P., da Fonseca, A. H., Angelo, I. da C., & Pinheiro, J. (2018). Effects of fluazuron on the biological parameters of engorged females of Rhipicephalus microplus. Brazilian Journal of Veterinary Medicine, 39(4), 231–238. https://doi.org/10.29374/2527-2179.bjvm023017


The objective of this study was to evaluate the biological parameters of Rhipicephalus microplus engorged females exposed to the arthropod growth regulator fluazuron. A commercial formulation based on fluazuron (Acatak®, Novartis) was administered topically to stabled cattle artificially infested with R. microplus larvae. The infestations occurred on four alternate days (days -21, -17, -13 and -6) taking as day 0 (zero) the day of treatment with a single dose of fluazuron for the treated group. Fifteen engorged females from each group were collected from the floor of the stalls at the end of the engorgement period (21 days) on pre-established days: before (day 0) and after treatment (days 4, 8 and 15), respectively. After cleaning and preparation of the engorged females in the laboratory, the following biological parameters were analyzed: weight of the engorged female, weight of the female after laying (spent female), laying period, weight of the posture, egg production index, reproductive efficiency index, hatching percentage, and nutritional index. The average weight of engorged females showed a significant reduction on all days after treatment in the exposed group. Aside from the weight after laying, all other biological parameters showed a significant reduction on the eighth post-treatment day due to exposure to fluazuron. Knowledge of the effects of an active principle on biological parameters in a population of ticks is essential for appropriate implementation of integrated management practices for parasite control.



Benetti, G.F. (1974). Oviposition of Boophilus microplus (Canestrini, 1987) (Acarina: Ixodidae). I. Influence of tick size on egg production. Acarologia, 16(1), 52-61. PMID: 4463680.

Borges, L. M. F., Carneiro, J. R. C., Gomes, A. G., & Moreira, P. C. (2001). Influência do peso inicial e da estação do ano na conversão em ovos de fêmeas de Boophilus microplus (Acari: Ixoxidae). Ciência Animal Brasileira, 2(2), 127-131.

Calligaris, I. B., Oliveira, P. R ., Roma, G. C., Bechara, G. H., & Camargo-Mathias, M. I. (2013). Action of the Insect Growth Regulator Fluazuron, The Active Ingredient of the Acaricide Acatak VR, in Rhipicephalus anguineusnymphs (Latreille, 1806) (Acari: Ixodidae). Microscopy Research and Technique, 76(11), 1177-1185. http://dx.doi.org/10.1002/jemt.22282. PMid:24000046.

Cohen, E. (1987). Chitin biochemistry: synthesis and inhibition. Annual Review of Entomology, 32(1), 71-93. http://dx.doi.org/10.1146/annurev.en.32.010187.000443.

Cordovés, C. O. (1997). Carrapato: control ou erradicação (1st ed., 176 p.). Guaíba: Agropecuária.

Cruz, B. C., Teixeira, W. F. P., Maciel, W. G ., Felippelli, G., Fávero, F. C ., Cruz, A. C., Buzzulini, C., Soares, V. E ., Gomes, L. V. C., Lopes, W. D. Z., Oliveira, G . P., & da Costa, A. J. (2014). Effects of fluazuron (2.5 mg/kg) and a combination of fluazuron (3.0 mg/kg) + abamectin (0.5 mg/kg) on the reproductive parameters of a field population of Rhipicephalus (Boophilus) microplus on experimentally infested cattle. Research in Veterinary Science, 97(1), 80-84. http://dx.doi.org/10.1016/j.rvsc.2014.04.012. PMid:24837997.

Furlong, J., & Sales, R. O. (2007). Control estratégico de carrapatos no bovino de leite: uma revisão. Revista Brasileira de Higiene e Sanidade Animal, 1(2), 44-72. http://dx.doi.org/10.5935/1981-2965.20070009.

Glória, M. A., Faccini, J. L. H., Daemon, E., & Grisi, L. (1993). Biologia comparativa da fase não parasitária de estirpes de B. microplus (Can., 1887) resistente e sensível a carrapaticida em condições de laboratório. Revista Brasileira de Parasitologia Veterinária, 2, 79-84.

Graf, J . - F. (1993). The role of insect growth regulators in arthropod control. Parasitology Today, 9(12), 471-474. http://dx.doi.org/10.1016/0169-4758(93)90106-P. PMid:15463697.

Grisi, L., Leite, R. C., Martins, J. R. S., Barros, A. T. M., Andreotti, R., Cançado, P. H. D., Léon, A. A. P., Pereira, J. B., & Villela, H. S. (2014). Reassessment of the potential economic impact of cattle parasites in Brazil. Revista Brasileira de Parasitologia Veterinária, 23(2), 150-156. http://dx.doi.org/10.1590/S1984-29612014042. PMid:25054492.

Klowden, M. J. (2007). Integumentary systems. In M. J. Klowden. Physiological systems in insects (Chapter 2, 2nd ed., pp. 75-135). London: Academic Press.

Meléndez, R. D., Coronado, A., Mujica, F., Cerutti, F., & Mosquera, O. (1998). Levels of natural resistance two Boophilus microplus (Acari: Ixodidae) in Corora breed bulls. Revista de Biología Tropical, 46(3), 691-696. PMid:10347818.

Oliveira, P. R ., Calligaris, I. B., Nunes, P. H ., Bechara, G. H., & Camargo-Mathias, M. I. (2014). Fluazuron-induced morphological changes in Rhipicephalus sanguineus Latreille, 1806 (Acari: Ixodidae) nymphs: An ultra-structural evaluation of the cuticle formationand digestive processes. Acta Tropica, 133, 45-55. http://dx.doi.org/10.1016/j.actatropica.2014.01.008. PMid:24508101.

Oliveira, P. R ., Calligaris, I. B., Roma, G. C., Bechara, G. H., Pizano, M. A., & Mathias, M. I. C. (2012). Potential of the insect growth regulator, fluazuron, in the control of Rhipicephalus sanguineus nymphs (Latreille, 1806) (Acari: Ixodidae): Determination of the LD95 and LD50. Experimental Parasitology, 131(1), 35-39. http://dx.doi.org/10.1016/j.exppara.2012.02.023. PMid:22465612.

Palli, S. R., & Retnakaran, A. 1999. Molecular and biochemical aspects of chitin synthesis inhibition (pp. 85-98). In P. Jolles, R. A. A. Muzzarelli (Eds.), Chitin and Chitinases. Basel: Birka user Verlag. http://dx.doi.org/10.1007/978-3-0348-8757-1_6.

Pereira, M. C., Labruna, M. B., Szabó, M. P. J., & Klafke, G. M. (2008). Rhipicephalus (Boophilus) microplus. Biologia, control e resistência (1ª ed., 169 p.). São Paulo: Med Vet.Sonenshine, D. E. (1991). The biology of ticks. New York: Oxford University Press.Souza, G. S. (2009). Avaliação da atividade do novaluron, sobre Boophilus microplus (Canestrini) em bovinos de corte naturalmente infestados (Dissertação de mestrado). Universidade Católica de Goiás, Goiânia.

Tatchell, R. J., & Schuntner, C. A. (1972). Glucose metabolism in the cattle tick Boophilus microplus.Journal of Insect Physiology, 18(2), 283-288. http://dx.doi.org/10.1016/0022-1910(72)90128-X.

Vasuki, V.; Rajavel, A. R. (1992). Influence of short time exposure to an insect growth regulator, Hexaflumuron, on mortality and adult emergence of vector mosquitoes. Memórias do Instituto Oswaldo Cruz, 87(2), 275-283.

Willadsen, P., Kemp, D. H., & Mckenna, R . V. (1984). Bloodmeal ingestion, and utilization as a component of host specificity in the tick, Boophilus microplus.Zeitschrift for Parasitenkunde, 70(3), 415-420. http://dx.doi.org/10.1007/BF00927829.

World Health Organization – WHO. (1997). Toxicological evaluation of certain veterinary drug residues in food. Geneva: International Programme on Chemical Safety.World Health Organization – WHO. (1998). Residues of some veterinary drugs in animals and foods (48-55). Rome.