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Abstract
This report describes two cases involving the isolation of Acinetobacter spp. and Proteus mirabilis from aborted ovine fetuses in Northeastern Brazil, representing the first documented occurrence of this association worldwide. In case 1, Acinetobacter spp. was isolated from a hepatic abscess in a White Dorper fetus, accompanied by placental lesions. In case 2, A. baumannii and P. mirabilis were isolated from the brain and lungs of three Santa Inês fetuses, with pathological findings consistent with acute septicemia. In both cases, bacteria were identified through culture and biochemical testing, without molecular confirmation, and no laboratory testing was conducted to exclude classical abortifacients (Brucella spp., Campylobacter spp., Coxiella burnetii, Chlamydia abortus, Listeria spp., Salmonella spp., Toxoplasma gondii, and Neospora caninum), representing a study limitation. Nevertheless, the results highlight the potential opportunistic role of these microorganisms, the detection of antimicrobial resistance profiles, and the importance of including them in the investigation of reproductive losses, particularly in settings with limited diagnostic resources. These findings reinforce the need for integrated surveillance in animal and public health under the “One Health” approach.
References
Abdollahi, M., Javan, A. J., Shokrpoor, S., Beidokhtinezhad, M., & Tamai, I. A. (2022). Pyoderma caused by Proteus mirabilis in sheep. Veterinary Medicine and Science, 8(6), 2562-2567. http://doi.org/10.1002/vms3.926. PMid:36049140.
Ali, T., Kamran, K., Raziq, A., Wazir, I., Ullah, R., Shah, P., Ali, M. I., Han, B., & Liu, G. (2021). Prevalence of mastitis pathogens and antimicrobial susceptibility of isolates from cattle and buffaloes in Northwest of Pakistan. Frontiers in Veterinary Science, 8, 746755. http://doi.org/10.3389/fvets.2021.746755. PMid:34722707.
Ahuatzin-Flores, O. E., Torres, E., & Chávez-Bravo, E. (2024). Acinetobacter baumannii, a multidrug-resistant opportunistic pathogen in new habitats: A systematic review. Microorganisms, 12(4), 644. http://doi.org/10.3390/ microorganisms12040644. PMid:38674589.
Akter, R., El-Hage, C. M., Sansom, F. M., Carrick, J., Devlin, J. M., & Legione, A. R. (2021). Metagenomic investigation of potential abortigenic pathogens in foetal tissues from Australian horses. BMC Genomics, 22(1), 713. http://doi.org/10.1186/s12864-021-08010-5. PMid:34600470.
Arserim, N. B., Sever, N. K., Unver, O., Keskin, O., & Ozcan, F. (2020). A Case of Acinetobacter lwoffii in an aborted calf. International Journal of Scientific and Technological Research, 6(13), 12-15. http://doi.org/10.7176/JSTR/6-13-02.
Askari, N., Momtaz, H., & Tajbakhsh, E. (2019). Acinetobacter baumannii in sheep, goat, and camel raw meat: Virulence and antibiotic resistance pattern. AIMS Microbiology, 5(3), 272-284. http://doi.org/10.3934/ microbiol.2019.3.272. PMid:31663061.
Baran, M. R., Negri Filho, L. C., Chineze, P. H. N., Bronkhorst, D. E., Pereira, C. E. S., Bogado, A. L. G., da Silva, L. C., Marcasso, R. A., & Okano, W. (2015). Proteus mirabilis como causa de afecção renal associada com alterações patológicas em touro Nelore: relato de caso. Revista Brasileira de Higiene e Sanidade Animal, 9(1), 78-90. http://doi.org/10.5935/1981-2965.20150008.
Botes, H. J. W. (1964). Proteus mirabilis as a cause of disease in calves. Journal of the South African Veterinary Association, 35(3), 385-391. https://journals.co.za/doi/pdf/10.10520/AJA00382809_1100
Chakkour, M., Hammoud, Z., Farhat, S., El Roz, A., Ezzeddine, Z., & Ghssein, G. (2024). Overview of Proteus mirabilis pathogenicity and virulence. Insights into the role of metals. Frontiers in Microbiology, 15, 1383618. http://doi.org/10.3389/fmicb.2024.1383618. PMid:38646633.
Concha-Bermejillo, A., & Romano, J. (2021). Pregnancy loss in small ruminants. Clinical Theriogenology, 13(3), 194-205.
Chen, Q., Zhou, W., Cheng, Y., Wang, G., San, Z., Guo, L., Liu, L., Zhao, C., & Sun, N. (2024). Four novel Acinetobacter lwoffii strains isolated from the milk of cows in China with subclinical mastitis. BMC Veterinary Research, 20(1), 274. http://doi.org/10.1186/s12917-024-04119-3. PMid:38918815.
Das, A. M., & Paranjape, V. L. (1986). Acinetobacter calcoaceticus in three cases of late abortion in water buffaloes. The Veterinary Record, 118(8), 214. http://doi.org/10.1136/vr.118.8.214. PMid:3716168.
Diker, K. S., Arda, M., & İzgür, H. (1986). Isolation of Acinetobacter calcoaceticus from cows with metritis. Zentralblatt für Veterinärmedizin. Reihe B., 33(8), 632-633. http://doi.org/10.1111/j.1439-0450.1986.tb00079.x. PMid:3799102.
Dorsch, M. A., Francia, M. E., Tana, L. R., González, F. C., Cabrera, A., Calleros, L., Sanguinetti, M., Barcellos, M., Zarantonelli, L., Ciuffo, C., Maya, L., Castells, M., Mirazo, S., da Silva Silveira, C., Rabaza, A., Caffarena, R. D., Doncel Díaz, B., Aráoz, V., Matto, C., Armendano, J. I., Salada, S., Fraga, M., Fierro, S., & Giannitti, F. (2022). Diagnostic investigation of 100 cases of abortion in sheep in Uruguay: 2015–2021. Frontiers in Veterinary Science, 9(1), 904786. http://doi.org/10.3389/fvets.2022.904786. PMid:35664842.
Elbehiry, A., Marzouk, E., Moussa, I. M., Dawoud, T. M., Mubarak, A. S., Al-Sarar, D., Alsubki, R. A., Alhaji, J. H., Hamada, M., Abalkhail, A. A., Hemeg, H., & Zahran, R. N. (2021). Acinetobacter baumannii as a community foodborne pathogen: Peptide mass fingerprinting analysis, genotypic of biofilm formation and phenotypic pattern of antimicrobial resistance. Saudi Journal of Biological Sciences, 28(1), 1158-1166. http://doi.org/10.1016/j.sjbs.2020.11.052. PMid:33424412.
El Damaty, H. M., ElDemerdash, A. S., Abd ElAziz, N. K., Yousef, S. G., Hefny, A. A., Abo Remela, E. M., Shaker, A., & Elsohaby, I. (2023). Molecular characterization and antimicrobial susceptibilities of Corynebacterium pseudotuberculosis isolated from caseous lymphadenitis of smallholder sheep and goats. Animals, 13(14), 2337. http://doi.org/10.3390/ani13142337. PMid:37508114.
Eroksuz, Y., Otlu, B., Eroksuz, H., Gursoy, N. C., Yerlikaya, Z., Akdeniz Incili, C., Karabulut, B., Timurkaan, N., & Timurkan, M. O. (2018). Brain abscess and bronchopneumonia caused by Acinetobacter baumannii in a 2-year-old female sheep. The Veterinary Quarterly, 38(1), 67-71. http://doi.org/10.1080/01652176.2018.1489165. PMid:30375283.
Garcia, G. G., Belotindos, L. P., & Mingala, C. N. (2013). Molecular characterization of respiratory bacterial pathogens in large and small ruminantes. Wetchasan Sattawaphaet, 43(4), 483-489. http://doi.org/10.56808/2985-1130.2512.
Howard, A., O’Donoghue, M., Feeney, A., & Sleator, R. D. (2012). Acinetobacter baumannii: An emerging opportunistic pathogen. Virulence, 3(3), 243-250. http://doi.org/10.4161/viru.19700. PMid:22546906.
Isogami, H., Sugeno, M., Imaizumi, K., Fukuda, T., Kamo, N., Yasuda, S., Yamaguchi, A., & Fujimori, K. (2023). Maternal septic shock due to Acinetobacter lwoffii infection: A case report. Fukushima Journal of Medical Science, 69(3), 191-196. http://doi.org/10.5387/fms.2022-43. PMid:37766560.
Lafta, M. H. (2020). Acute buffalo mastitis caused by mixed infection of Enterobacter cloacae and Proteus mirabilis at Basrah, Iraq. Magallat al-Basrat Li-l-Abhat al-Baytariyyat, 19(2), 130-141. http://doi.org/10.23975/ bjvetr.2020.174160.
Mellace, M., Ceniti, C., Paonessa, M., Procopio, A. C., & Tilocca, B. (2024). Multidrug-resistant Acinetobacter baumannii: An underestimated pathogen in veterinary medicine in Italy. German Journal of Veterinary Research, 4(2), 112-126. http://doi.org/10.51585/gjvr.2024.2.0089.
Müller, S., Janssen, T., & Wieler, L. H. (2014). Multidrug resistant Acinetobacter baumannii in veterinary medicine-emergence of an underestimated pathogen? Berliner und Munchener Tierarztliche Wochenschrift, 127(11-12), 435-446. PMid:25872253.
Najd Ghahremani, A., Abdollahi, M., Shokrpoor, S., & Ashrafi Tamai, I. (2023). Pericarditis caused by Proteus mirabilis in sheep. Veterinary Medicine and Science, 9(4), 1737-1741. http://doi.org/10.1002/vms3.1160. PMid:37192480.
Rahman, H., & Baxi, K. K. (1985). Isolation of Acinetobacter calcoaceticus from a cow with mastitis. Zentralblatt für Veterinärmedizin. Reihe B., 32(1), 71-72. http://doi.org/10.1111/j.1439-0450.1985.tb01939.x. PMid:3976314.
Silva, V. F., Damasceno, T. E. F., Souza, N. J. D., Franco, I., & Costa, M. M. (2011). Microbiota cérvico-vaginal de ovelhas mestiças e sua susceptibilidade aos antibióticos. Pesquisa Veterinária Brasileira, 31(7), 586-590. http://doi.org/10.1590/S0100-736X2011000700007.
Wolf-Jäckel, G. A., Hansen, M. S., Larsen, G., Holm, E., Agerholm, J. S., & Jensen, T. K. (2020). Diagnostic studies of abortion in Danish cattle 2015-2017. Acta Veterinaria Scandinavica, 62(1), 1. http://doi.org/10.1186/s13028- 019-0499-4. PMid:31900210.
Zappa, V., Bolaños, C. A. D., Paula, C. L., Callefe, J. L. R., Alves, A. C., Morais, A. B. C., Guerra, S. T., Cabrini, M. C., Melville, P. A., & Ribeiro, M. G. (2017). Antimicrobial multiple resistance index, minimum inhibitory concentrations, and extended-spectrum beta-lactamase producers of Proteus mirabilis and Proteus vulgaris strains isolated from domestic animals with various clinical manifestations of infection. Semina: Ciências Agrárias, 38(2), 775-790. http://doi.org/10.5433/1679-0359.2017v38n2p775.
Whitman, W. B., Goodfellow, M., Kämpfer, P., Busse, H. J., Trujillo, M. E., Ludwig, W., & Suzuki, K. I. (Eds.) (2012). Bergey’s manual of systematic bacteriology: the actinobacteria (2nd ed., Vol. 5). New York: Springer. http://doi.org/10.1007/978-0-387-68233-4
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Copyright (c) 2026 Huber Rizzo, Urias Fagner Santos Nascimento, Márcio Douglas Leal da Silveira, Emmylly Victória Gomes de Lima, Lorena Gabriela Rocha Ribeiro, Erika Fernanda Torres Samico-Fernandes, Valdemiro Amaro da Silva Junior