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Abstract
This study evaluated the effects of two milk replacer-based diets, one supplemented with starter feed and the other with alfalfa hay, on rumen development in preweaned Holstein calves. Twenty newborn male calves were fed 8 L/day of milk replacer plus either starter feed (group A) or alfalfa hay (group B) ad libitum for 10 weeks. Rumen morphometry and histological features were assessed via gross anatomical evaluation, histology, and scanning electron microscopy. The calves in group A consumed significantly more solid feed and exhibited greater papillae development, with higher papillae height, width, and density per cm2, than those in group B. Moreover, the surface enlargement factor was consistently greater in group A across ruminal regions, except in the caudodorsal blind sac (saccus cecus caudodorsalis; p < 0.05). Empty rumen weight was also higher in starter-fed calves, suggesting enhanced mucosal development and absorptive capacity. Notably, keratin layer thickness did not explain the observed differences between the groups. Despite similar final body weights and growth, calves supplemented with starter feed showed greater rumen papillae development and absorptive surface area than those supplemented with alfalfa hay. These findings suggest that starter feeding during high milk allowance promotes epithelial maturation and enhances post-weaning absorption of fermentation products.
References
Antúnez-Tort, G., Bach, A., Cajarville, C., Vidal, M., Fàbregas, F., Ahangarani, M. A., & Terré, M. (2023). Benefits of barley straw as a forage for dairy calves before and after weaning. Journal of Dairy Science, 106(11), 7578-7590. https://doi.org/10.3168/jds.2023-23401. PMid:37558048.
Association of Official Analytical Chemists – AOAC. (2000). Official methods of analysis of AOAC International (17th ed.). Rockville: AOAC International.
Beharka, A. A., Nagaraja, T. G., Morrill, J. L., Kennedy, G. A., & Klemm, R. D. (1998). Effects of form of the diet on anatomical, microbial, and fermentative development of the rumen of neonatal calves. Journal of Dairy Science, 81(7), 1946-1955. https://doi.org/10.3168/jds.S0022-0302(98)75768-6. PMid:9710764.
Brown, E. G., VandeHaar, M. J., Daniels, K. M., Liesman, J. S., Chapin, L. T., Keisler, D. H., & Nielsen, M. W. (2005a). Effect of increasing energy and protein intake on body growth and carcass composition of heifer calves. Journal of Dairy Science, 88(2), 585-594. https://doi.org/10.3168/jds.S0022-0302(05)72722-3. PMid:15653525.
Brown, E. G., VandeHaar, M. J., Daniels, K. M., Liesman, J. S., Chapin, L. T., Forrest, J. W., Akers, R. M., Pearson, R. E., & Nielsen, M. W. (2005b). Effect of increasing energy and protein intake on mammary development in heifer calves. Journal of Dairy Science, 88(2), 595-603. https://doi.org/10.3168/jds.S0022-0302(05)72723-5. PMid:15653526.
Brownlee, A. (1956). The development of rumen papillae in cattle fed on different diets. The British Veterinary Journal, 112(9), 369-375. https://doi.org/10.1016/S0007-1935(17)46456-6.
Clauss, M., Fritz, J., Bayer, D., Nygren, K., Hammer, S., Hatt, J. M., Südekum, K. H., & Hummel, J. (2009a). Physical characteristics of rumen contents in four large ruminants of different feeding type, the addax (Addax nasomaculatus), bison (Bison bison), red deer (Cervus elaphus) and moose (Alces alces). Comparative Biochemistry and Physiology. Part A, Molecular & Integrative Physiology, 152(3), 398-406. https://doi.org/10.1016/j.cbpa.2008.11.009. PMid:19049897.
Clauss, M., Hofmann, R. R., Fickel, J., Streich, W. J., & Hummel, J. (2009b). The intraruminal papillation gradient in wild ruminants of different feeding types: Implications for rumen physiology. Journal of Morphology, 270(8), 929-942. https://doi.org/10.1002/jmor.10729. PMid:19247992.
Cozzi, G., Gottardo, F., Mattiello, S., Canali, E., Scanziani, E., Verga, M., & Andrighetto, I. (2002). The provision of solid feeds to veal calves: I. Growth performance, forestomach development, and carcass and meat quality. Journal of Animal Science, 80(2), 357-366. https://doi.org/10.2527/2002.802357x. PMid:11881925.
Deelen, S. M., Ollivett, T. L., Haines, D. M., & Leslie, K. E. (2014). Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves. Journal of Dairy Science, 97(6), 3838-3844. https://doi.org/10.3168/jds.2014-7939. PMid:24704239.
Diao, Q., Zhang, R., & Fu, T. (2019). Review of strategies to promote rumen development in calves. Animals, 9(8), 490. https://doi.org/10.3390/ani9080490. PMid:31357433.
Drackley, J. K. (2008). Calf nutrition from birth to breeding. The Veterinary Clinics of North America. Food Animal Practice, 24(1), 55-86. https://doi.org/10.1016/j.cvfa.2008.01.001. PMid:18299032.
Flatt, W. P., Warner, R. G., & Loosli, J. K. (1959). Evaluation of several techniques used in the study of developing rumen function. Journal of Dairy Science, (Suppl.), 361.
Hinders, R. G., & Owen, F. G. (1965). Relation of ruminal parakeratosis development to volatile fatty acid absorption. Journal of Dairy Science, 48(8), 1069-1073. https://doi.org/10.3168/jds.S0022-0302(65)88393-X. PMid:5891234.
Khan, M. A., Weary, D. M., & Von Keyserlingk, M. A. G. (2011). Hay intake improves performance and rumen development of calves fed higher quantities of milk. Journal of Dairy Science, 94(7), 3547-3553. https:// doi.org/10.3168/jds.2010-3871. PMid:21700042.
Lagger, J. (2010). Crecimiento intensivo de cría y recría de vaquillonas, aplicando los principios de bienestar. Revista Veterinaria Argentina, 27(265), 1-28.
Licitra, G., Hernandez, T. M., & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57(4), 347-358. https://doi.org/10.1016/0377-8401(95)00837-3.
National Research Council – NRC. (2001). Nutrient requirements of dairy cattle. Washington, D.C.: National Academy Press.
Nocek, J. E., & Kesler, E. M. (1980). Growth and rumen characteristics of Holstein steers fed pelleted or conventional diets. Journal of Dairy Science, 63(2), 249-254. https://doi.org/10.3168/jds.S0022-0302(80)82921-3.
Nocek, J. E. (1997). Bovine acidosis: Implications on laminitis. Journal of Dairy Science, 80(5), 1005-1028. https:// doi.org/10.3168/jds.S0022-0302(97)76026-0. PMid:9178142.
Owens, F. N., Dubeski, P., & Hanson, C. F. (1993). Factors that alter the growth and development of ruminants. Journal of Animal Science, 71(11), 3138-3150. https://doi.org/10.2527/1993.71113138x. PMid:8270538.
Rickard, M. D., & Ternouth, J. H. (1965). The effect of increased dietary volatile fatty acids on the morphological and physiological development of lambs with particular reference to the rumen. Journal of Agricultural Science, 65(3), 371-377. https://doi.org/10.1017/S0021859600048930.
Scocco, P., Ceccarelli, P., Gatti, R., & Catorci, A. (2007). Use of a geographic information system to evaluate morphometric variations of rumen papillae related to diet and pasture vegetative cycle. Veterinaria Italiana, 43(3), 425-429. PMid:20422518.
Stobo, I. J. F., Roy, J. H. B., & Gaston, H. J. (1966). Rumen development in the calf: 2. The effect of diets containing different proportions of concentrates to hay on digestive efficiency. The British Journal of Nutrition, 20(2), 189-215. https://doi.org/10.1079/BJN19660022. PMid:5938700.
Suárez, B. J., Van Reenen, C. G., Gerrits, W. J. J., Stockhofe, N., Van Vuuren, A. M., & Dijkstra, J. (2006). Effects of supplementing concentrates differing in carbohydrate composition in veal calf diets: II. Rumen development. Journal of Dairy Science, 89(11), 4376-4386. https://doi.org/10.3168/jds.S0022-0302(06)72484-5. PMid:17033025.
Suárez, B. J., Van Reenen, C. G., Stockhofe, N., Dijkstra, J., & Gerrits, W. J. J. (2007). Effect of roughage source and roughage to concentrate ratio on animal performance and rumen development in veal calves. Journal of Dairy Science, 90(5), 2390-2403. https://doi.org/10.3168/jds.2006-524. PMid:17430943.
Tamate, H., McGilliard, A. D., Jacobson, N. L., & Getty, R. (1962). Effect of various dietaries on the anatomical development of the stomach in the calf. Journal of Dairy Science, 45(3), 408-420. https://doi.org/10.3168/jds. S0022-0302(62)89406-5.
Terler, G., Velik, M., Poier, G., Sener-Aydemir, A., Klevenhusen, F., & Zebeli, Q. (2023). Feeding concentrate with hay of different qualities modulates rumen histology, development of digestive tract as well as slaughter performance and meat quality of young dairy calves. Archives of Animal Nutrition, 77(3), 171-186. https:// doi.org/10.1080/1745039X.2023.2197633. PMid:37255000.
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. https:// doi.org/10.3168/jds.S0022-0302(91)78551-2. PMid:1660498.
Vazquez, N., Santos, D., Antúnez, G., Amaro, N., Escardó, J., López, L., & Cajarville, C. (2025). Are there differences in abomasal morphology in calves fed eight liters of milk and concentrate or good quality alfalfa hay? Brazilian Journal of Veterinary Medicine, 47, e002825. https://doi.org/10.29374/2527-2179.bjvm002825. PMid:41035544.
Vieira, A. D. P., Guesdon, V., De Passillé, A. M., Von Keyserlingk, M. A. G., & Weary, D. M. (2008). Behavioural indicators of hunger in dairy calves. Applied Animal Behaviour Science, 109(2–4), 180-189. https:// doi.org/10.1016/j.applanim.2007.03.006.
Webb, L. E., Engel, B., Berends, H., van Reenen, C. G., Gerrits, W. J., de Boer, I. J., & Bokkers, E. A. (2014). What do calves choose to eat and how do preferences affect behaviour? Applied Animal Behaviour Science, 161, 7-19. https://doi.org/10.1016/j.applanim.2014.09.016.
Weigand, E., Young, J. W., & McGilliard, A. D. (1975). Volatile fatty acid metabolism by rumen mucosa from cattle fed hay or grain. Journal of Dairy Science, 58(9), 1294-1300. https://doi.org/10.3168/jds.S0022-0302(75)84709- 6. PMid:1184811.
Yohe, T. T., Dennis, T. S., Buss, L. N., Croft, E. J. D., Quigley, J. D., Hill, T. M., Suárez-Mena, F. X., Aragona, K. M., Laarman, A. H., Costa, J. H. C., & Steele, M. A. (2022). Performance and visceral tissue growth and development of Holstein calves fed differing milk replacer allowances and starch concentrations in pelleted starter. Journal of Dairy Science, 105(5), 4099-4115. https://doi.org/10.3168/jds.2021-21286. PMid:35221069.
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Copyright (c) 2026 Noelia Vazquez, Dellis dos Santos, Rody Artigas, Germán Antúnez, Nicolás Amaro, Mario López, Richard Mas, Cecilia Cajarville