Abstract
This study investigated the anatomical and histological characteristics of the abomasum in weaned Holstein calves under two feeding regimens: high-quality alfalfa hay and starter concentrate. The calves were raised under an accelerated feeding system (milk diet provided at 20% of their body weight). This study aimed to determine the effects of diet on the development of the abomasum during the early life stages of calves, which is crucial for digesting both milk and solid feed. Twenty newborn male Holstein calves were randomly assigned to either diet group and monitored from 56 to 60 days of age. Organ weight, abomasal curvature length, and number of abomasal folds were measured. Histological slides were prepared for microscopic examination. No significant differences in anatomical and histological parameters were observed between the two dietary groups, suggesting that both diets provided adequate nutritional support for development. Although the quantity of feed differed, the primary component, milk, remained consistent, contributing to balanced nutrition. This study emphasized the importance of early nutrition in calves and its influence on the anatomical and histological development of the abomasum. However, potential subtle differences warrant further investigation to determine long-term consequences on digestive health and efficiency.
References
Aage, H. M., Bhosale, N. S., Kadam, S. D., & Kapadnis, P. J. (2007). Histomorphological study of abomasum in cattle. Indian Journal of Animal Research, 41(3), 192-195.
Ali, M. A., Alhasson, F. A., & Shehan, N. A. (2023). Anatomy, histological, patho-physiological comparative study of abomasums disorders in cows and buffaloes (a review study). Central Asian Journal of Medical and Natural Sciences, 4(5), 653-660.
Amaro, N., Campbell, V., Dayuto, J., Antúnez, G., & Cajarville, C. (2024). High-quality forage as only solid feed for unweaned dairy calves on accelerated rearing. In Proceedings of the ADSA Annual Meeting, West Palm Beach, Florida. Champaign, IL: ADSA.
Antúnez-Tort, G., Bach, A., Cajarville, C., Vidal, M., Fàbregas, F., Ahangarani, M. A., & Terré, M. (2023). Benefits of barley straw as a forage for dairy calves before and after weaning. Journal of Dairy Science, 106(11), 7578-7590. http://doi.org/10.3168/jds.2023-23401. PMid:37558048.
Association of Analytical Communities – AOAC. (2000). Official methods of analysis of AOAC International (17th ed.). Gaithersburg, MD: AOAC.
Burgstaller, J., Wittek, T., & Smith, G. W. (2017). Invited review: Abomasal emptying in calves and its potential influence on gastrointestinal disease. Journal of Dairy Science, 100(1), 17-35. http://doi.org/10.3168/jds.2016-10949. PMid:27865497.
Davis, C. L., & Drackley, J. K. (1998). The development, nutrition, and management of the young calf. Ames: Iowa State University Press.
Deelen, S. M., Ollivett, T. L., Haines, D. M., & Leslie, K. E. (2014). Evaluation of a Brix refractometer to estimate serum immunoglobulin G concentration in neonatal dairy calves. Journal of Dairy Science, 97(6), 3838-3844. http://doi.org/10.3168/jds.2014-7939. PMid:24704239.
Diao, Q., Zhang, R., & Fu, T. (2019). Review of strategies to promote rumen development in calves. Animals, 9(8), 490. http://doi.org/10.3390/ani9080490. PMid:31357433.
Drackley, J. K. (2008). Calf nutrition from birth to breeding. The Veterinary Clinics of North America. Food Animal Practice, 24(1), 55-86. http://doi.org/10.1016/j.cvfa.2008.01.001. PMid:18299032.
Dyce, K. M., Sack, W. O., & Wensing, C. J. G. (2009). Textbook of veterinary anatomy. St Louis: Elsevier Health Sciences.
Khan, M. A., Bach, A., Weary, D. M., & von Keyserlingk, M. A. G. (2016). Invited review: Transitioning from milk to solid feed in dairy heifers. Journal of Dairy Science, 99(2), 885-902. http://doi.org/10.3168/jds.2015-9975. PMid:26709160.
Kia, H. D., Karimi, H., Hosseinkhani, A., Rafat, S. A., & Tghizadeh, A. (2016). histological effects of different levels of sorghum grain on the abomasum, duodenum and colon of Ghezel× Arkhar-Merino crossbred lambs. Animal and Veterinary Sciences, 4(5), 72. http://doi.org/10.11648/j.avs.20160405.11.
Lagger, J. (2010). Crecimiento intensivo de cría y recría de vaquillonas, aplicando los principios de bienestar. Veterinaria Argentina, 27(265), 1-28.
Lauwers, H., Ooms, L., Simoens, P., & De Vos, N. (1979). The functional structure of the pylorus in the ox. Anatomia, Histologia, Embryologia, 8(1), 56-71. http://doi.org/10.1111/j.1439-0264.1979.tb00679.x. PMid:157085.
Lavín, P., & Mantecón, A. R. (1993). Fisiología digestiva de los rumiantes salvajes. Zamora: Colegio Oficial de Veterinarios de Zamora. http://hdl.handle.net/10261/23030
Licitra, G., Hernandez, T. M., & Van Soest, P. J. (1996). Standardization of procedures for nitrogen fractionation of ruminant feeds. Animal Feed Science and Technology, 57(4), 347-358. http://doi.org/10.1016/0377-8401(95)00837-3.
Mahesh, R., Singh, G., & Kumar, P. (2017). Light microscopic studies on the abomasum of goat (Capra hircus). Veterinary Research International, 5(1), 21-27.
Mbatha, K. R., Downs, C. T., & Nsahlai, I. V. (2002). The effects of graded levels of dietary tannin on the epithelial tissue of the gastro-intestinal tract and liver and kidney masses of Boer goats. Animal Science, 74(3), 579-586. http://doi.org/10.1017/S1357729800052735.
Movahedizadeh, M., Raji, A. R., & Mirshahi, A. (2018). Histomorphometric and ultrasonographic evaluations of the abomasum in sheep. Comparative Clinical Pathology, 27(4), 855-859. http://doi.org/10.1007/s00580-018-2673-x.
National Research Council – NRC. (2001). Nutrient requirements of dairy cattle (7th ed.). Washington: National Academy.
Nicholls, C. D., Lee, D. L., & Sharpe, M. J. (1985). Scanning electron microscopy of biopsy specimens removed by a colonoscope from the abomasum of sheep infected with Haemonchus contortus. Parasitology, 90(2), 357-363. http://doi.org/10.1017/S0031182000051052.
Quigley, J. (1997). Palatabilidad de los iniciadores para terneros (Nota Acerca de Terneros, No. 47). Calf Notes. http://calfnotes.com/pdffiles/CN047e.pdf.
Raeth-Knight, M., Chester-Jones, H., Hayes, S., Linn, J., Larson, R., Ziegler, D., Ziegler, B., & Broadwater, N. (2009). Impact of conventional or intensive milk replacer programs on Holstein heifer performance through six months of age and during first lactation. Journal of Dairy Science, 92(2), 799-809. http://doi.org/10.3168/jds.2008-1470. PMid:19164694.
Raji, A. R. (2011). Morphological and histochemical investigation of the camel (Camelus dromedarius) abomasal mucous membrane by light and scanning electron microscopy (SEM). Majallah-i Tahqiqat-i Dampizishki-I Iran, 12(4), 304-308. http://doi.org/10.22099/ijvr.2011.81.
Sarma, K., & Bhattacharya, M. (1997). Comparative gross anatomy of abomasum of mithun (Bos frontalis), yak (Bos grunniens) and zebu (Bos indicus). The Indian Journal of Animal Sciences, 67(10), 876-877.
Terré, M., Devant, M., & Bach, A. (2007). Effect of level of milk replacer fed to Holstein calves on performance during the preweaning period and starter digestibility at weaning. Livestock Science, 110(1-2), 82-88. http://doi.org/10.1016/j.livsci.2006.10.001.
Van Soest, P. J., Robertson, J. B., & Lewis, B. A. (1991). Methods for dietary fiber, neutral detergent fiber, and nonstarch polysaccharides in relation to animal nutrition. Journal of Dairy Science, 74(10), 3583-3597. http://doi.org/10.3168/jds.S0022-0302(91)78551-2. PMid:1660498.

This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2025 Noelia Vazquez, Dellis dos Santos, Germán Antúnez, Nicolás Amaro, Josefina Escardó, Lucía López, Cecilia Cajarville