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Abstract
The current study was aimed at assessing the status of vitamin D3 in growing Najdi lambs in Basra province, Iraq. By using a model of musculoskeletal illness, “Najdi lambs”, sera were examined for determining the levels of the total serum vitamin D3, Ca (calcium), P (phosphorus), PTH (parathyroid hormone), ALP (alkaline phosphatase), and ALT (Alanine aminotransferase).The results showed that there was a sharp down-regulation (17.7 ± 1.07 Ng/ML) of total serum vitamin D3 in Najdi lambs that had signs of musculoskeletal disorders, poor body and hair condition scores, decrease appetite, and poor growth when compared to high levels (89.75 ± 7.06 Ng/ML) in control healthy Najdi lambs. However, dark coat color Najdi lambs had higher serum levels (P<0.05) of vitamin D3 than light coat color Najdi lambs in both deficient to vitamin D3 and control healthy lambs. Interestingly, a correlation between the total serum vitamin D3 concentration and the serum level of Ca/P ratio, PTH, ALP and ALT was observed. Thus in lambs with low vitamin D3 concentration, Ca and P concentrations were lower while PTH, ALP and ALT were higher than in lambs with higher concentrations of vitamin D3. In the final consideration, deficiency of vitamin D3 in local Najdi lambs potentially threatens their health and has an important role in suppressing their immunity. Our study provides evidence for association of the pigmentation and the metabolism of vitamin D3. Evaluation the serum levels of reliable minerals, hormones and enzymes asbiomarkers can support the early diagnosis of metabolic diseases included vitamin D3 in domestic animals.
References
Alabada, H. K. M., & Saleh, W. M. M. (2020). Vitamin D effectiveness and pathology in humans and domestic animals. Multidisciplinary Reviews, 3(1), e2020010. http://doi.org/10.29327/multi.2020010.
Alamer, M., & Al-hozab, A. (2004). Effect of water deprivation and season on feed intake, body weight and thermoregulation in Awassi and Najdi sheep breeds in Saudi Arabia. Journal of Arid Environments, 59(1), 71-84. http://doi.org/10.1016/j.jaridenv.2004.01.003.
Altug, N., Agaoglu, Z., Yuksek, N., Kaya, A., & Keleş, İ. (2006). Adenosine deaminase in the diagnosis of white muscle diseases in lambs. Medycyna Weterynaryjna, 62, 1007-1010.
Amanoel, D., Thomas, D., Blache, D., Milton, J., Wilmot, M., Revell, D., & Norman, H. (2016). Sheep deficient in vitamin E preferentially select for a feed with a higher concentration of vitamin E. Animal, 10(2), 183-191. http://doi.org/10.1017/S1751731115001937. PMid:26355660.
Bioassay Technology Laboratory. (2023). Shanghai. https://www.bt-laboratory.com/
Bouillon, R., & Suda, T. (2014). Vitamin D: Calcium and bone homeostasis during evolution. BoneKEy Reports, 480, 3. http://doi.org/10.1038/bonekey.2013.214. PMid:24466411.
Cleal, J. K., Hargreaves, M. R., Poore, K. R., Tang, J. C., Fraser, W. D., Hanson, M. A., & Green, L. R. (2017). Reduced fetal vitamin D status by maternal undernutrition during discrete gestational windows in sheep. Journal of Developmental Origins of Health and Disease, 8(3), 370-381. http://doi.org/10.1017/S2040174417000149. PMid:28327211.
Constable, P., Hinchcliff, K., Done, S., & Gruenberg, W. (2017). A textbook of the diseases of cattle, horses, sheep, pigs, and goats (Vol. 11). Madrid: WB Saunders.
Craig, L., Dittmer, K., & Thompson, K. (2016). Pathology of domestic animals (6th ed.). St. Louis: Elsevier.
Demay, M. B. (2006). Mechanism of vitamin D receptor action. Annals of the New York Academy of Sciences, 1068(1), 204-213. http://doi.org/10.1196/annals.1346.026. PMid:16831920.
Denizhan, V., Kozat, S., & Yilmaz, A. (2019). Investigation of serum Amyloid A, haptoglobin and fibrinogen levels in sheep infected with Fasciola hepatica. Journal of Livestock Science, 10(-), 53-58. http://doi.org/10.33259/ JLivestSci.2019.53-58.
Dittmer, K., & Thompson, K. (2011). Vitamin D metabolism and rickets in domestic animals: A review. Veterinary Pathology, 48(2), 389-407. http://doi.org/10.1177/0300985810375240. PMid:20634407.
Handel, I., Watt, K. A., Pilkington, J. G., Pemberton, J. M., Macrae, A., Scott, P., McNeilly, T. N., Berry, J. L., Clements, D. N., Nussey, D. H., & Mellanby, R. J. (2016). Vitamin D status predicts reproductive fitness in a wild sheep population. Scientific Reports, 6(1), 18986. http://doi.org/10.1038/srep18986. PMid:26757805.
Holick, M. F. (1981). The cutaneous photosynthesis of previtamin D3: A unique photoendocrine system. The Journal of Investigative Dermatology, 77(1), 51-58. http://doi.org/10.1111/1523-1747.ep12479237. PMid:6265564.
Holick, M. F. (2004). Sunlight and vitamin D for bone health and prevention of autoimmune diseases, cancers, and cardiovascular disease. The American Journal of Clinical Nutrition, 80(6, Suppl), 1678S-1688S. http://doi.org/10.1093/ajcn/80.6.1678S. PMid:15585788.
Holick, M. F., Binkley, N. C., Bischoff-Ferrari, H. A., Gordon, C. M., Hanley, D. A., Heaney, R. P., Murad, M. H., & Weaver, C. M. (2012). Guidelines for preventing and treating vitamin D deficiency and insufficiency revisited. The Journal of Clinical Endocrinology and Metabolism, 97(4), 1153-1158. http://doi.org/10.1210/jc.2011-2601. PMid:22442274.
Horst, R., & Littledike, E. (1982). Comparison of plasma concentrations of vitamin D and its metabolites in young and aged domestic animals. Comparative Biochemistry and Physiology. B, Comparative Biochemistry, 73(3), 485-489. http://doi.org/10.1016/0305-0491(82)90064-5. PMid:6983948.
Hrkovic-Porobija, A., Hodzic, A., & Hadzimusic, N. (2017). Functional liver stress in dairy sheep. Indian Journal of Small Ruminants, 23(2), 194-197. http://doi.org/10.5958/0973-9718.2017.00060.5.
Jackson, M. L. (2013). Veterinary clinical pathology: An introduction. Ames: John Wiley & Sons.
Khan, S. A., Ahmed, N., & Tunio, M. T. (2019). Incidence of liver fluke infestation and pathological examination in sheep (Ovis aries) in Mirpur Azad Jammu and Kashmir. Pure and Applied Biology, 8(1), 750-761. http://doi.org/10.19045/bspab.2019.80017.
Majeed, S., Ramadhan, M., & Monther, W. (2013). Long-term toxicological effects of paracetamol in rats. Iraqi Journal of Veterinary Sciences, 27(1), 65-70. http://doi.org/10.33899/ijvs.2013.82959.
Mearns, R., Scholes, S., Wessels, M., Whitaker, K., & Strugnell, B. (2008). Rickets in sheep flocks in northern England. The Veterinary Record, 162(3), 98-99. http://doi.org/10.1136/vr.162.3.98. PMid:18204038.
Nelson, C. D., Lippolis, J. D., Reinhardt, T. A., Sacco, R. E., Powell, J. L., Drewnoski, M. E., O’Neil, M., Beitz, D. C., & Weiss, W. P. (2016). Vitamin D status of dairy cattle: Outcomes of current practices in the dairy industry. Journal of Dairy Science, 99(12), 10150-10160. http://doi.org/10.3168/jds.2016-11727. PMid:27743666.
Nisbet, D., Butler, E., Smith, B., Robertson, J., & Bannatyne, C. (1966). Osteodystrophic diseases of sheep. 2. Rickets in young sheep. Journal of Comparative Pathology, 76(2), 159-169. http://doi.org/10.1016/0021-9975(66)90018- 1. PMid:6006080.
Norman, A. W. (2011). Vitamin D nutrition is at a crossroads. Public Health Nutrition, 14(4), 744-745. http://doi.org/10.1017/S1368980011000280. PMid:21435285.
Porobija, A. H., Hodzic, A., Ohran, H., Vegara, M., Rustempašic, A., & Velic, L. (2018). Biochemical and endocrine profiling of Bosnia and Hercegovina’s Native Pramenka sheep. Journal of Animal Research, 8(5), 741-750. http://doi.org/10.30954/2277-940X.10.2018.2.
Pugh, D. G., & Baird, N. N. (2012). Sheep & goat medicine (Vol. 2). St. Louis: Elsevier.
Radostits, O. M., Gay, C. C., Hinchcliff, K. W., & Constable, P. D. (2006). Veterinary medicine: A textbook of the diseases of cattle, horses, sheep, pigs and goats. London: Elsevier Health Sciences.
Radostits, O., Gay, C., Hinchcliff, K., Constable, P. (2007). Veterinary medicine: A textbook of the diseases of cattle, sheep, pigs, goats and horses (Vol. 10). Philadelphia: Elsevier.
Saleh, W. M. M., Ibrahim, A. A., Alhamed, T. A. A., Habib, H. N., Naeem, R. M., Alabada, H. K. M., & Abdulrasool, A. M. (2020). Efficacy of oral administration of a reliable AD3E treatment on vitamin D3 deficiency in Najdi sheep. IOP Conference Series. Materials Science and Engineering, 928(6), 062028. http://doi.org/10.1088/1757-899X/928/6/062028.
Saleh, W., Lafta, M., Abdulrazaq, A., Habib, H., & Naeem, L. (2019). Bacteriological and histopathological evaluation of infectious lymphadenitis caused by pseudomonas aeruginosa in awasi sheep. Advances in Animal and Veterinary Sciences, 7(5), 378-382. http://doi.org/10.17582/journal.aavs/2019/7.5.378.382.
Thomas, M. K., & Demay, M. B. (2000). Vitamin D deficiency and disorders of vitamin D metabolism. Endocrinology and Metabolism Clinics of North America, 29(3), 611-627, viii. http://doi.org/10.1016/S0889-8529(05)70153-5. PMid:11033763.
Uhl, E. W. (2018). The pathology of vitamin D deficiency in domesticated animals: An evolutionary and comparative overview. International Journal of Paleopathology, 23, 100-109. http://doi.org/10.1016/j.ijpp.2018.03.001. PMid:29544996.
Uyar, A., Yener, Z., Basbugan, Y., Keles, O. F., & Yaman, T. (2017). Examination of sheep in which injection of Yeldif® induced sudden death. Medycyna Weterynaryjna, 73(8), 473-478. http://doi.org/10.21521/mw.5759.
van Saun, R. J. (2004). Vitamin D-responsive rickets in neonatal lambs. The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 45(10), 841-844. PMid:15532884.
Weber, G., Witschi, A., Wenk, C., & Martens, H. (2014). Effects of dietary 25-hydroxycholecalciferol and cholecalciferol on blood vitamin D and mineral status, bone turnover, milk composition, and reproductive performance of sows. Journal of Animal Science, 92(3), 899-909. http://doi.org/10.2527/jas.2013-7209. PMid:24492559.
Zhou, P., McEvoy, T. G., Gill, A. C., Lambe, N. R., Morgan-Davies, C. R., Hurst, E., Sargison, N. D., & Mellanby, R. J. (2019). Investigation of relationship between vitamin D status and reproductive fitness in Scottish hill sheep. Scientific Reports, 9(1), 1162. http://doi.org/10.1038/s41598-018-37843-6. PMid:30718917.
Zongping, L. (2005). Studies on rickets and osteomalacia in Bactrian camels (Camelus bactrianus). Veterinary Journal, 169(3), 444-453. http://doi.org/10.1016/j.tvjl.2004.03.014. PMid:15848787.
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Copyright (c) 2025 Wessam Monther Mohammed Saleh, Hayder Kamil Maryoosh Alabada, Rafid Majeed Naeem, Israa Abdulwadood Alsaad, Hussein Ali Naji