Assessing muscle function and oxidative profile in criollo horses participating in long loop rodeo before and after exercise
PDF
XML

Keywords

biochemical changes, long loop competition, equine, exercise, oxidative stress.

How to Cite

Pereira, A. T., Pozzobon, R., dos Anjos, B. L., Chaves, L. T., Smilgys, Érika C., Thoma, A. J. S., … Soares, J. C. M. (2024). Assessing muscle function and oxidative profile in criollo horses participating in long loop rodeo before and after exercise. Brazilian Journal of Veterinary Medicine, 46, e005824. https://doi.org/10.29374/2527-2179.bjvm005823

Abstract

Criollo breed is quite popular in Brazil, mainly in the Southern Region, and the long loop rodeo is one of the main sports modality adapted to this breed. The purpose of this study was to assess changes in the muscle and oxidative functions caused by exercises practiced in this sports modality. Data such as exercising frequency, covered distance, mean duration time and number of runs, and blood samples at the following times: before the tournament, on the final day of the event, and 18–24 h after the end of the tournament were collected. Were assessed lactate and the enzymes aspartate aminotransferase and creatine kinase (for the evaluation of the muscle function) and species reactive to oxygen, species reactive to thiobarbituric acid, catalase, superoxide dismutase and uric acid (for oxidative stress). Data were assessed through variance analysis associated with Tukey test (P≤0.05), as well as through Spearman’s correlation (P≤0.05). Significant creatine kinase and lactate increase in T1 associated with the maintenance of oxidative indicator levels in T1 and T18 reflected the significant muscle effort and the slight mobilization of oxidative parameters, which were compensated by the antioxidant defenses, since the assessed animals recovered after T18. There was no damage at cell level and the horses showed good muscle and antioxidant condition for exercising.

https://doi.org/10.29374/2527-2179.bjvm005823
PDF
XML

References

Amaral, L. A., Torres, A., Rabassa, V., Martins, C. F., Correa, M. N., & Nogueira, C. E. W. (2013). Limiar anaeróbico (V4) e frequência cardíaca de cavalos Crioulos condicionados para prova funcional. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 65(1), 181-188. http://doi.org/10.1590/S0102-09352013000100027.

Andriichuk, A., Tkachenko, H., & Tkachova, I. (2016). Oxidative stress biomarkers and erythrocytes hemolysis in well-trained equine athletes before and after exercise. Journal of Equine Veterinary Science, 36, 32-43. http://doi.org/10.1016/j.jevs.2015.09.011.

Associação Brasileira de Criadores de Cavalos Crioulos – ABCCC. (2018). Regulamento do registro genealógico da raça equina Crioula. https://www.cavalocrioulo.org.br/studbook/regulamentos

Binda, M. B., Teixeira, F. A., Carvalho, R. S., Macedo, L. P., Conti, L. M. C., Manso Filho, H. C., & Coelho, C. S. (2016). Effects of 3-barrel racing exercise on electrocardiographic and on blood parameters of quarter horses. Journal of Equine Veterinary Science, 47, 71-76. http://doi.org/10.1016/j.jevs.2016.08.001.

Braz, P. H., Ghetti, E. R. M. L., Sartoretto, M. C., & Deboleto, S. G. C. (2016). Avaliações séricas de glicose, lactato, creatina quinase e aspartato aminotransferase em equinos quarto de milha antes e depois de prova de corrida. Acta Veterinaria Brasilica, 10, 322-326.

Chiaradia, E., Avellini, L., Rueca, F., Spaterna, A., Porciello, F., Antonioni, M. T., & Gaiti, A. (1998). Physical exercise, oxidative stress and muscle damage in racehorses. Comparative Biochemistry and Physiology. Part B, Biochemistry & Molecular Biology, 119(4), 833-836. http://doi.org/10.1016/S0305-0491(98)10001-9. PMid:9787774.

Da Cás, E. L., Rosauro, A. C., Silva, C. A. M., & Brass, K. E. (2000). Concentração sérica das ezimas creatinoquinase, aspartato aminotransferase e dehidrogenase lática em equinos raça Crioula. Ciência Rural, 30(4), 625. http://doi.org/10.1590/S0103-84782000000400011.

Deaton, C. M., & Marlin, D. J. (2003). Exercise-associated oxidative stress. Clinical Techniques in Equine Practice, 2(3), 278-291. http://doi.org/10.1053/S1534-7516(03)00070-2.

Di Filippo, P. A., Martins, L. P., Meireles, M. A. D., Lannes, S. T., Peçanha, R. M. S., & Graça, F. A. S. (2016). Gender differences - induced changes in serum hematologic and biochemical variables in Mangalarga Marchador horses after a marcha gait competition. Journal of Equine Veterinary Science, 43, 18-22. http://doi.org/10.1016/j.jevs.2016.04.093.

Fazio, F., Casella, S., Assenza, A., Arfuso, F., Tosto, F., & Piccione, G. (2014). Blood biochemical changes in show jumpers during a simulated show jumping test. Veterinarski Arhiv, 84(2), 143-152.

Franciscato, C., Lopes, S. T. A., Veiga, A. P. M., Martins, D. B., Emanuelli, M. P., & Oliveira, L. S. S. (2006). Atividade sérica das enzimas AST, CK e GGT em cavalos Crioulos. Pesquisa Agropecuária Brasileira, 41(10), 1561-1565. http://doi.org/10.1590/S0100-204X2006001000014.

Gama, J. A. N., Souza, M. S., Pereira Neto, E., Souza, V. R. C., & Coelho, C. S. (2013). Concentrações séricas de aspartato aminotransferase e creatinoquinase e concentrações plasmáticas de lactato em equinos da raça Mangalarga Marchador após exercício físico. Brazilian Journal of Veterinary Research and Animal Science, 49(6), 480-486. http://doi.org/10.11606/issn.1678-4456.v49i6p480-486.

Gaschler, M. M., & Stockwell, B. R. (2017). Lipid peroxidation in cell death. Biochemical and Biophysical Research Communications, 482(3), 419-425. http://doi.org/10.1016/j.bbrc.2016.10.086. PMid:28212725.

Halliwell, B., & Chirico, S. (1993). Lipid peroxidation: Its mechanism, measurement, and significance. The American Journal of Clinical Nutrition, 57(5, Suppl), 715S-725S. http://doi.org/10.1093/ajcn/57.5.715S. PMid:8475889.

Jentzsch, A. M., Bachmann, H., Fürst, P., & Biesalski, H. K. (1996). Improved analysis of malondialdehyde in human body fluids. Free Radical Biology & Medicine, 20(2), 251-256. http://doi.org/10.1016/0891-5849(95)02043-8. PMid:8746446.

Kolanjiappan, K., Manoharan, S., & Kayalvizhi, M. (2002). Measurement of erythrocyte lipids, lipid peroxidation, antioxidants and osmotic fragility in cervical cancer patients. Clinica Chimica Acta, 326(1-2), 143-149. http://doi.org/10.1016/S0009-8981(02)00300-5. PMid:12417105.

Landgraf Botteon, P. T. L. (2012). Lactato na medicina veterinária: Atualização conceitual. Brazilian Journal of Veterinary Medicine, 34(4), 283-287.

McCord, J. M., & Fridovich, I. (1969). Superoxide dismutase an enzymic function for erythrocuprein (hemocuprein). The Journal of Biological Chemistry, 244(22), 6049-6055. http://doi.org/10.1016/S0021-9258(18)63504-5. PMid:5389100.

Moffarts, B., Kirschvink, N., Art, T., Pincemail, J., Michaux, C., Cayeux, K., Defraigne, J., & Lekeux, P. (2004). Impact of training and exercise intensity on blood antioxidant markers in healthy Standardbred horses. Equine Comp Exerc Physiol, 1(3), 211-220. http://doi.org/10.1079/ECP200426.

Myhre, O., Andersen, J. M., Aarnes, H., & Fonnum, F. (2003). Evaluation of the probes 2′,7′-dichlorofluorescin diacetate, luminol, and lucigenin as indicators of reactive species formation. Biochemical Pharmacology, 65(10), 1575-1582. http://doi.org/10.1016/S0006-2952(03)00083-2. PMid:12754093.

Nelson, D. L., & Kiesow, L. A. (1972). Enthalpy of decomposition of hydrogen peroxide by catalase at 25 °C (with molar extinction coefficients of H2O2 solutions in the UV). Analytical Biochemistry, 49(2), 474-479. http://doi.org/10.1016/0003-2697(72)90451-4. PMid:5082943.

Rose, R. J., & Hodgson, D. R. (1994). An overview of performance and sport medicine. In D. R. Hodgson & R. J. Rose (Eds.), The athletic horse (pp. 5-11). Philadelphia: W. B. Saunders.

Schneider, C. D., & Oliveira, A. R. (2004). Radicais livres de oxigênio e exercício: Mecanismos de formação e adaptação ao treinamento físico. Revista Brasileira de Medicina do Esporte, 10(4), 308-313. http://doi.org/10.1590/ S1517-86922004000400008.

Sies, H. (1986). Biochemistry of oxidative stress. Angewandte Chemie International Edition in English, 25(12), 1058-1071. http://doi.org/10.1002/anie.198610581.

Soares, J. C. M., Zanela, R., Bondan, C., Alves, L. P., Lima, M. R., Motta, A. C., & & Zanella, E. L. (2011). Biochemical and antioxidante changes in plasma, serum and erythrocites of horses before and after a jumping competition. Journal of Equine Veterinary Science, 31(7), 357-360. http://doi.org/10.1016/j.jevs.2011.03.017.

Soares, O. A. B., Ferraz, G. C., Martins, C. B., Dias, D. P. M., Lacerda-Neto, J. C., & Queiroz-Neto, A. (2014). Comparison of maximal lactate steady state with V2, V4, individual anaerobic threshold and lactate minimum speed in horses. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 66(1), 39-46. http://doi.org/10.1590/ S0102-09352014000100007.

Valko, M., Leibfritz, D., Moncol, J., Cronin, M. T., Mazur, M., & Telser, J. (2007). Free radicals and antioxidants in normal physiological functions and human disease. The International Journal of Biochemistry & Cell Biology, 39(1), 44-84. http://doi.org/10.1016/j.biocel.2006.07.001. PMid:16978905.

Vasconcelos, S. M. L., Goulart, M. O. F., Moura, J. B. F., Benfato, M. S., Manfredini, V., & Kubota, L. T. (2007). Espécies reativas de oxigênio e de nitrogênio, antioxidantes e marcadores de dano oxidativo em sangue humano: Principais métodos analíticos para sua determinação. Química Nova, 30(5), 1323-1338. http://doi.org/10.1590/ S0100-40422007000500046.

Vidart, D. (2004). Orígenes del Caballo Criollo. In L. Ponce de León (Ed.), Criollos de America: Origen y evolucion de uma raza legendaria (pp. 15-23). Uruguai: Ponce de León y Zorrilla.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2024 Andrielli Trentim Pereira, Ricardo Pozzobon, Bruno Leite dos Anjos, Leonardo Trentin Chaves, Érika Carla Smilgys, Ana Júlia Santos Thoma, Vinícius Leobet Lunkes, Juliana Sorraila de Oliveira, Cinthia Melazzo de Andrade, Júlio Cesar Mendes Soares