Abstract
This study aimed to assess the fluctuations of antibody serum titers for Toxoplasma gondii and Neospora caninum in naturally infected crossbred cows during gestation and to investigate transplacental transmission of T. gondii; 51 cows were monitored during pregnancy by monthly serologic testing by indirect fluorescent antibody test. 33 cows were seronegative for both N. caninum and T. gondii, 10 were seropositive only for N. caninum, 5 were seropositive only for T. gondii, and 3 were seropositive for both N. caninum and T. gondii. In both protozoan infections, great variation in antibody levels in pregnant cows was observed, and there was significant increase (p<0.05) in the comparison between the averages of serological titration per trimester. There was a significant correlation (p<0.05) between month and probability of seropositivity for T. gondii. We conclude that pregnancy influences antibody titers of crossbred cows naturally infected with N. caninum and/or T. gondii, and that serologic testing for T. gondii in pregnant cows from the sixth month of gestation onward may decrease the number of false negative results.
References
Antonello, A. M., Camillo, G., Weber, A., Braunig, P., Sangioni, L. A., & Vogel, F. S. F. (2015). Dinâmica sorológica de anticorpos contra Neospora caninum durante a gestação de vacas naturalmente infectadas. Ciência Animal Brasileira, 16(4), 553-559. http://dx.doi.org/10.1590/1089-6891v16i428127.
Bates, D., Machler, M., Bolker, B., & Walker, S. (2015). Fitting linear mixed-effects models using lme4. Journal of Statistical Software, 67(1), 1-48. http://dx.doi.org/10.18637/jss.v067.i01.
Bergeron, N., Fecteau, G., Paré, J., Martineau, R., & Villeneuve, A. (2000). Vertical and horizontal transmission of Neospora caninum in dairy herds in Québec. The Canadian Veterinary Journal. La Revue Veterinaire Canadienne, 41(6), 464-467. PMid:10857030.
Cabral, A. D., Camargo, C. N., Galleti, N. T. C., Okuda, L. H., Pituco, E. M., & Del Fava, C. (2013). Screening for Toxoplasma gondii in aborted bovine fetuses in Brazil. Arquivos do Instituto Biológico, 280(1), 103-105. http://dx.doi.org/10.1590/S1808-16572013000100015.
Camargo, M. E. (1964). Improved technique of indirect immunofluorescence for serological diagnosis of toxoplasmosis. Revista do Instituto de Medicina Tropical de São Paulo, 6(3), 117-118. PMid:14177810.
Cardoso, J. M. S., Nishi, S. M., Funada, M. R., Amaku, M., Guimarães Júnior, J. S., & Gennari, S. M. (2009). Antibody dynamics during gestation in cows naturally infected with Neospora caninum from four dairy herds in Brazil. Braz. Brazilian Journal of Veterinary Research and Animal Science, 46(5), 395-399. http://dx.doi.org/10.11606/issn.1678-4456.bjvras.2009.26789.
Costa, G. H., Costa, A. J., Lopes, W. D., Bresciani, K. D., Santos, T. R., Esper, C. R., & Santana, A. E. (2011). Toxoplasma gondi: Infection natural congenital in cattle and an experimental inoculation of gestating cows with oocysts. Experimental Parasitology, 127(1), 277-281. http://dx.doi.org/10.1016/j.exppara.2010.08.005. PMid:20736009.
Dijkstra, T., Barkema, H. W., Björkman, C., & Wouda, W. (2002). A high rate of seroconversion for Neospora caninum in a dairy herd without an obvious increased incidence of abortions. Veterinary Parasitology, 109(3-4), 203- 211. http://dx.doi.org/10.1016/S0304-4017(02)00303-5. PMid:12423933.
Dubey, J. P., Jenkins, M. C., Adams, D. S., McAllister, M. M., Anderson-Sprecher, R., Baszler, T. V., Kwok, O. C. H., Lally, N. C., Björkman, C., & Uggla, A. (1997). Antibody responses of cows during an outbreak of neosporosis evaluated by indirect fluorescent antibody test and different enzyme-linked immunosorbent assays. The Journal of Parasitology, 83(6), 1063-1069. http://dx.doi.org/10.2307/3284363. PMid:9406780.
Dubey, J. P., Miller, N. L., & Frenkel, J. K. (1970). The Toxoplasma gondii oocyst from cat feces. The Journal of Experimental Medicine, 132(4), 636-662. http://dx.doi.org/10.1084/jem.132.4.636. PMid:4927658.
Esteban-Redondo, I., & Innes, E. A. (1997). Toxoplasma gondii infection in sheep and cattle. Comparative Immunology, Microbiology and Infectious Diseases, 20(2), 191-196. http://dx.doi.org/10.1016/S0147-9571(96)00039- 2. PMid:9208205.
Frenkel, J. K., Dubey, J. P., & Miller, N. L. (1970). Toxoplasma gondii in cats: Fecal stages identified as coccidian oocysts. Science, 167(3919), 893-896. http://dx.doi.org/10.1126/science.167.3919.893. PMid:4903651.
Galvão, G. S., Gondim, L. F. P., Pereira, M. J. S., Oliveira, U. V., & Munhoz, A. D. (2011). Soropositividade para Neospora caninum e associacão ao abortamento e natimortos em rebanhos leiteiros do sudeste da Bahia. Brasil. Revista Brasileira de Medicina Veterinária, 33(4), 234-237.
Garcia, J. L., Marques, F. A. C., Vidotto, O., Navarro, I. T., Martins, G. F., Zulpo, D. L., Cunha, I. A. L., Taroda, A., Cardim, S. T., & Ewald, M. P. C. (2012). Soro ocorrência de anticorpos contra Toxoplasma gondii e transmissão vertical em vacas de corte (Bos indicus) abatidas. Semina: Ciências Agrárias, 33(3), 1095-1102. http://dx.doi.org/10.5433/1679-0359.2012v33n3p1095.
Garcia, J. L., Navarro, I. T., Ogawa, L., & Oliveira, R. C. (1999). Soroprevalência do Toxoplasma gondii em suínos, bovinos, ovinos e eqüinos, e sua correlação com humanos, felinos e caninos, oriundos de propriedades rurais do norte do Paraná, Brasil. Ciência Rural, 29(1), 91-97. http://dx.doi.org/10.1590/S0103-84781999000100017.
Gondim, L. F. P., McAllister, M. M., Pitt, W. C., & Zemlicka, D. E. (2004). Coyotes (Canis latrans) are definitive hosts of Neospora caninum. International Journal for Parasitology, 34(2), 159-161. http://dx.doi.org/10.1016/j.ijpara.2004.01.001. PMid:15037103.
Gondim, L. F. P., Pinheiro, A. M., Santos, P. O. M., Jesus, E. E. V., Ribeiro, M. B., Fernandes, H. S., Almeida, M. A., Freire, S. M., Meyer, R., & McAllister, M. M. (2001). Isolation of Neospora caninum from the brain of a naturally infected dog, and production of encysted bradyzoites in gerbils. Veterinary Parasitology, 101(1), 1-7. http://dx.doi.org/10.1016/S0304-4017(01)00493-9. PMid:11587828.
González-Warleta, M., Castro-Hermida, J. A., Carro-Corral, C., Cortizo-Mella, J., & Mezo, M. (2008). Epidemiology of neosporosis in dairy cattle in Galicia (NW Spain. Parasitology Research, 102(2), 243-249. http://dx.doi.org/10.1007/s00436-007-0753-y. PMid:17899194.
Gottstein, B., Hentrich, B., Wyss, R., Thür, B., Busato, A., Stärk, K. D., & Müller, N. (1998). Molecular and immunodiagnostic investigations on bovine neosporosis in Switzerland. International Journal for Parasitology, 28(4), 679-691. http://dx.doi.org/10.1016/S0020-7519(98)00006-X. PMid:9602392.
Hasler, B., Hernandez, J. A., Reist, M., Sager, H., Steiner-Moret, C., Staubli, D., Stärk, K. D., & Gottstein, B. (2006). Neospora caninum: Serological follow-up in dairy cows during pregnancy. Veterinary Parasitology, 137(3-4), 222-230. http://dx.doi.org/10.1016/j.vetpar.2006.01.007. PMid:16469449.
Kul, O., Kabakci, N., Yildiz, K., Ocal, N., Kalender, H., & Ilkme, N. A. (2009). Neospora caninum associated with epidemic abortions in dairy cattle: the first clinical neosporosis report in Turkey. Veterinary Parasitology, 159(1), 69-72. http://dx.doi.org/10.1016/j.vetpar.2008.10.019. PMid:19028014.
Lenth, R. V. (2016). Least-squares means: the R package lsmeans. Journal of Statistical Software, 69(1), 1-33. http:// dx.doi.org/10.18637/jss.v069.i01.
Macedo, M. F. S. B., Macedo, C. A. B., Barros, L. D., Martins, G. F., Sandeski, L. M., Zulpo, D. L., Cunha, I. A. L., Taroda, A., Cardim, S. T., Su, C., & Garcia, J. L. (2012a). Serum occurrence of anti-Toxoplasma gondii antibodies in dairy cows slaughtered in an abattoir for human consume. Ciência Rural, 42(6), 1065-1069. http://dx.doi.org/10.1590/S0103-84782012000600019.
Macedo, M. F. S. B., Macedo, C. A. B., Ewald, M. P. C., Martins, G. F., Zulpo, D. L., Cunha, I. A. L., Taroda, A., Cardim, S. T., Su, C., & Garcia, J. L. (2012b). Isolation and genotyping of Toxoplasma gondii from pregnant dairy cows (Bos taurus) slaughtered. Revista Brasileira de Parasitologia Veterinária, 21(1), 74-77. http://dx.doi.org/10.1590/S1984-29612012000100016. PMid:22534951.
Magalhães, V. C. S., Oliveira, U. V., Costa, S. C. L., Santos, I. A., Pereira, M. J. S., & Munhoz, A. D. (2014). Transmission paths of Neospora caninum in a dairy herd of crossbred cattle in the northeast of Brazil. Veterinary Parasitology, 202(3-4), 257-264. http://dx.doi.org/10.1016/j.vetpar.2014.01.018. PMid:24560936.
McAllister, M. M., Dubey, J. P., Lindsay, D. S., Jolley, W. R., Wills, R. A., & McGuire, A. M. (1998). Dogs are definitive hosts of Neospora caninum. International Journal for Parasitology, 28(9), 1473-1478. http://dx.doi.org/10.1016/ S0020-7519(98)00138-6. PMid:9770635.
Munhoz, A. D., Jacintho, A. P. P., & Machado, R. Z. (2011). Bovine abortion associated with Neospora caninum: Diagnosis and epidemiological aspects of a dairy cattle herd in the Northeast region of São Paulo State, Brazil. Brazilian Journal of Veterinary Pathology, 4(2), 112-116.
Nogareda, C., López-Gatius, F., Santolaria, P., García-Ispierto, I., Bech-Sábat, G., Pabón, M., Mezo, M., GonzalezWarleta, M., Castro-Hermida, J. A., Yániz, J., & Almeria, S. (2007). Dynamics of anti-Neospora caninum antibodies during gestation in chronically infected dairy cows. Veterinary Parasitology, 148(3-4), 193-199. http://dx.doi. org/10.1016/j.vetpar.2007.06.032. PMid:17643822.
Opsteegh, M., Teunis, P., Zuchner, L., Koets, A., Langelaar, M., & Van der Giessen, J. (2011). Low predictive value of seroprevalence of Toxoplasma gondii in cattle for detection of parasite DNA. International Journal for Parasitology, 41(3-4), 343-354. http://dx.doi.org/10.1016/j.ijpara.2010.10.006. PMid:21145321.
R Core Team. (2015). R: A Language and Environment for Statistical Computing. http://www.R-project.org/
Tenter, A. M. (2009). Toxoplasma gondii in animals used for human consumption. Memorias do Instituto Oswaldo Cruz, 104(2), 364-369. http://dx.doi.org/10.1590/S0074-02762009000200033. PMid:19430665.
Wiengcharoen, J., Thompson, R. C., Nakthong, C., Rattanakorn, P., & Sukthana, Y. (2011). Transplacental transmission in cattle: Is Toxoplasma gondii less potent than Neospora caninum? Parasitology Research, 108(5), 1235-1241. http://dx.doi.org/10.1007/s00436-010-2172-8. PMid:21203773.
Wouda, W., Moen, A. R., & Schukken, Y. H. (1998). Abortion risk in progeny of cows after a Neospora caninum epidemic. Theriogenology, 49(7), 1311-1316. http://dx.doi.org/10.1016/S0093-691X(98)00078-8. PMid:10732068.
Yamane, I., Kokuho, T., Shimura, K., Eto, M., Shibahara, T., Haritani, M., Ouchi, Y., Sverlow, K., & Conrad, P. A. (1997). In vitro isolation and characterization of a bovine Neospora species in Japan. Research in Veterinary Science, 63(1), 77-80. http://dx.doi.org/10.1016/S0034-5288(97)90162-4. PMid:9368961.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2023 Uillians Volkart de Oliveira, Vanessa Carvalho Sampaio de Magalhães, Sônia Carmen Lopo Costa, Ivan Bezerra Allaman, Alexandre Dias Munhoz