Evaluation of the susceptibility to fipronil of Rhipicephalus microplus larvae from egg masses incubated at different times of oviposition


in vitro test, laboratory colonies, tick.

How to Cite

Rocha, D. de O., Roque, R. M. da S., Vieira, T. de S., Guimarães, B. G., de Jesus, I. L. R., Rocha, M. B. da S., Scott, F. B., & Avelar, B. R. (2023). Evaluation of the susceptibility to fipronil of Rhipicephalus microplus larvae from egg masses incubated at different times of oviposition . Brazilian Journal of Veterinary Medicine, 45, e005922. https://doi.org/10.29374/2527-2179.bjvm005922


The objective of this work was to evaluate the susceptibility of R. microplus larvae from different oviposition times to fipronil. The LPT was performed in sextuplicate, at concentrations of 18.75, 37.5, 75, 150 and 300 μg.mL-1. The LC50 found for the egg masses incubated with +7, +14 and +21 days were respectively 105.87, 110.71 and 121.22 μg.mL-1. The larvae originating from egg masses from the same group of engorged females, incubated on different days, presented similar mortality rates compared to the evaluated fipronil concentrations, facilitating the maintenance of laboratory colonies of this tick species.



Castro-Janer, E., Rifran, L., Piaggio, J., Gil, A., Miller, R. J., & Schumaker, T. T. S. (2009). In vitro tests to establish LC50 and discriminating concentrations for fipronil against Rhipicephalus (Boophilus) microplus (Acari: Ixodidae) and their standardization. Veterinary Parasitology, 162(1-2), 120-128. http://dx.doi.org/10.1016/j. vetpar.2009.02.013. PMid:19278787.

Davey, R. B., Garza Junior, J., Thompson, G. D., & Drummond, R. O. (1980). Ovipositional biology of the southern cattle tick, Boophilus microplus (Acari: Ixodidae), in the laboratory. Journal of Medical Entomology, 17(2), 117- 121. http://dx.doi.org/10.1093/jmedent/17.2.117.

Figueiredo, A., Nascimento, L. M., Lopes, L. G., Giglioti, R., Albuquerque, R. D. D. G., Santos, M. G., Falcão, D. Q., Nogueira, J. A. P., Rocha, L., & Chagas, A. C. S. (2018). First report of the effect of Ocotea elegans essential oil on Rhipicephalus (Boophilus) microplus. Veterinary Parasitology, 252, 131-136. http://dx.doi.org/10.1016/j.vetpar.2018.02.018. PMid:29559134.

Food and Agriculture Organization of the United Nations. (2004). Guidelines resistance management and integrated parasite control in ruminants. FAO.

Grisi, L., Leite, R. C., Martins, J. R. D. S., Barros, A. T. M. D., Andreotti, R., Cançado, P. H. D., & Villela, H. S. (2014). Reassessment of the potential economic impact of cattle parasites in Brazil. Revista Brasileira de Parasitologia Veterinária, 23(2), 150-156. http://dx.doi.org/10.1590/S1984-29612014042.

Hitchcock, L. F. (1955). Studies on the non-parasitic stages of the cattle tick, Boophilus microplus (Canestrini) (AcarinaL Ixodidae). Australian Journal of Zoology, 3(3), 295-311. http://dx.doi.org/10.1071/ZO9550295.

Pereira, M. D. C., Labruna, M. B., Szabó, M. P. J., & Klafke, G. M. (2008). Rhipicephalus (Boophilus) microplus: Biologia, controle e resistência. Editora Med Vet Livros.

Reck, J., Klafke, G. M., Webster, A., Dall’Agnol, B., Scheffer, R., Souza, U. A., Corassini, V. B., Vargas, R., dos Santos, J. S., & de Souza Martins, J. R. (2014). First report of fluazuron resistance in Rhipicephalus microplus: a field tick population resistant to six classes of acaricides. Veterinary Parasitology, 201(1-2), 128-136. http://dx.doi.org/10.1016/j.vetpar.2014.01.012. PMid:24560364.

Stone, B. F., & Haydock, K. P. (1962). A method for measuring the acaricide-susceptibility of the cattle tick Boophilus microplus (Can.). Bulletin of Entomological Research, 53(3), 563-578. http://dx.doi.org/10.1017/ S000748530004832X.

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Copyright (c) 2023 Daniela de Oliveira Rocha, Roxanne Marina da Silva Roque, Thiago de Souza Vieira, Brena Gava Guimarães, Ingrid Lins Raquel de Jesus, Marisa Beatriz da Silva Rocha, Fabio Barbour Scott, Barbara Rauta Avelar