Ozone therapy: protocol for treating canine parvovirus infection


integrative medicine, ozone therapy, parvovirus, canine.

How to Cite

dos Santos, T. G., Orlandin, J. R., de Almeida, M. F., Scassiotti, R. F., Oliveira, V. C., Santos, S. I. P., … Ambrósio, C. E. (2023). Ozone therapy: protocol for treating canine parvovirus infection . Brazilian Journal of Veterinary Medicine, 45, e004622. https://doi.org/10.29374/2527-2179.bjvm004622


Canine Parvovirus infection is a disease caused by Canine Parvovirus (CPV) that results in hemorrhagic gastroenteritis and secondary infections, mainly in puppies between six weeks and six months old that are not immunized. Since there is no specific treatment for the condition, supportive therapy based on antibiotics, antiemetics, and non-steroidal anti-inflammatory drugs is traditionally used. Ozone therapy is an economical treatment that has bactericidal, fungicidal, and antiviral properties, besides promoting oxygenation and tissue regeneration, as well as anti-inflammatory and analgesic effects, and was used as a complementary therapy in this study. Therefore, four mixed-breed dogs, aged between 2 and 3 months, with no previous immunization against CPV and testing positive for the virus in a rapid test were selected. The animals were randomly distributed into two groups, being 1: the control group (n=2) that received only supportive treatment; and 2: the experimental group (n=2), that in addition to conventional therapy received intravenously 500 mL of ozonized Ringer’s Lactate solution. Before treatment and after 24 and 48 hours, the following clinical signs were evaluated: episodes of emesis and diarrhea, weight, hydration, blood glucose level, abdominal pain, and blood count. One control group animal died within the first hours of hospitalization. Both animals in the experimental group presented faster resolution of diarrheal episodes and shorter hospitalization time when compared to the surviving animal that received only supportive treatment. Although further studies are needed, ozone therapy showed promising results for the treatment of canine parvovirus.



Alves, F. S., Alonso, F. H., Horta, R. S., Barbosa, B. C., Beier, S., & Paes, P. R. O. (2020). Prognostic values of physical and hematological parameters of dogs naturally infected with parvovirus PVC-2: Retrospective study of 103 cases. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 72(6), 2127-2134. http://dx.doi. org/10.1590/1678-4162-11517.

Ambrósio, C. E., Orlandin, J. R., Oliveira, V. C., Motta, L. C. B., Pinto, P. A. F., Pereira, V. M., Padoveze, L. R., Karam, R. G., & Pinheiro, A. O. (2020). Potential application of aminiotic stem cells in veterinary medicine. Animal Reproduction, 16(1), 24-30. http://dx.doi.org/10.21451/1984-3143-AR2018-0124. PMid:33299475.

Castro, T. X., Miranda, S. C., Labarthe, N. V., Silva, L. E., & Cubel Garcia, R. C. N. (2007). Clinical and epidemiological aspects of canine parvovirus (CPV) enteritis in the State of Rio de Janeiro: 1995-2004. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 59(2), 10. http://dx.doi.org/10.1590/S0102-09352007000200010.

Chumpitazi, B. P., Self, M. M., Czyzewski, D. I., Cejka, S., Swank, P. R., & Shulman, R. J. (2016). Bristol Stool Form Scale reliability and agreement decreases when determining Rome III stool form designations. Neurogastroenterology and Motility, 28(3), 443-448. http://dx.doi.org/10.1111/nmo.12738. PMid:26690980.

Faria, P. F., Araújo, D. F., & Soto-Blanco, B. (2005). Glicemia em cães obesos e senis. Acta Scientiae Veterinariae, 33(1), 47-50. http://dx.doi.org/10.22456/1679-9216.14446.

Feitosa, F. L. F. (Ed.). (2017). Semiologia veterinária: A arte do diagnóstico (3ª ed., 627 p.). São Paulo: Roca.

Ferreira, R. R., Barbosa, P. R., Godinho, E., Costa, U. M., Gonzalez Felix, H. D., & Ferreiro, L. (2004). Alterações hemato-bioquímicas em cães jovens com gastrenterite viral: relato de 18 casos. MEDVEP. Revista Científica de Medicina Veterinária. Pequenos Animais e Animais de Estimação, 2(7), 159-163.

Ghiggi, E., Padilha, V. S., Moraes, A. N., Lima, M. P. A., Gehrcke, M. I., Luiz, R. M., & Oleskovicz, N. (2013). Reposição volêmica com hidroxietilamido ou solução de ringer lactato em cães com gastroenterite hemorrágica por parvovírus. Semina: Ciências Agrárias, 34(4), 1783-1791. http://dx.doi.org/10.5433/1679-0359.2013v34n4p1783.

Glickman, L. T., Domanski, L. M., Patronek, G. J., & Visintainer, F. (1985). Breed-related risk factors for canine parvovirus enteritis. Journal of the American Veterinary Medical Association, 187(6), 589-594. PMid:3003015.

Haddad, M. A., Souza, M. V., Hincapie, J. J., Ribeiro Junior, J. I., Ribeiro Filho, J. D., & Benjamin, L. A. (2009). Comportamento de componentes bioquímicos do sangue em equinos submetidos à ozonioterapia. Arquivo Brasileiro de Medicina Veterinária e Zootecnia, 61(3), 539-546. http://dx.doi.org/10.1590/S0102-09352009000300003.

Hirschmann, L. C. (2012). Padrões hematológicos de cães errantes provenientes de seis municípios do Rio Grande do Sul [Monografia, Universidade Federal do Semi-Árido]. Porto Alegre.

Horecka, K., Porter, S., Amirian, E. S., & Jefferson, E. (2020). A decade of treatment of canine parvovirus in an animal shelter: A retrospective study. Animals, 10(6), 939. http://dx.doi.org/10.3390/ani10060939. PMid:32485882.

Kalli, I., Leontides, L. S., Mylonakis, M. E., Adamama-Moraitou, K., Rallis, T., & Koutinas, A. F. (2010). Factors affecting the occurrence, duration of hospitalization and final outcome in canine parvovirus infection. Research in Veterinary Science, 89(2), 174-178. http://dx.doi.org/10.1016/j.rvsc.2010.02.013. PMid:20303134.

Lopes, R. R. F. B., Quessada, A. M., Freire, L. D. S., Lima, W. C., Dantas Lima, D. A. S., Rodrigues, M. C., Sala, P. L., Landi, U. N., & Zaniolo, M. M. (2017). Leucócitos totais em cães com gastroenterite hemorrágica tratados por autohemoterapia. Jornal Interdisciplinar de Biociências, 2(1), 1. http://dx.doi.org/10.26694/2448-0002.vl2iss1pp1-5.

Mantione, N. L., & Otto, C. M. (2005). Characterization of the use of antiemetic agents in dogs with parvoviral enteritis treated at a veterinary teaching hospital: 77 Cases (1997-2000). Journal of the American Veterinary Medical Association, 227(11), 1787-1793. http://dx.doi.org/10.2460/javma.2005.227.1787. PMid:16342528.

Markovich, J. E., Stucker, K. M., Carr, A. H., Harbison, C. E., Scarlett, J. M., & Parrish, C. R. (2012). Effects of canine parvovirus strain variations on diagnostic test results and clinical management of enteritis in dogs. Journal of the American Veterinary Medical Association, 241(1), 66-72. http://dx.doi.org/10.2460/javma.241.1.66. PMid:22720989.

McCaw, D. L., & Hoskins, J. D. (2006). Canine viral enteritis. In C. E. Greene (Ed.), Infectious diseases of the dog and cat (2nd ed., pp. 63-73). Philadelphia: W.B. Saunders Elsevier.

Morais, M. P., & Costa, P. R. (2007). Parvoviridae. In E. F. Flores (Ed.), Virologia veterinária (888 p). Santa Maria: Ed. UFSM.

Orlandin, J. R., Machado, L. C., Ambrósio, C. E., & Travagli, V. (2021). Ozone and its derivatives in veterinary medicine: A careful appraisal. Veterinary and Animal Science, 13, 100191. http://dx.doi.org/10.1016/j.vas.2021.100191. PMid:34401601.

Pereira, G. Q., Gomes, L. A., Santos, I. S., Alfieri, A. F., Weese, J. S., & Costa, M. C. (2018). Fecal microbiota transplantation in puppies with canine parvovirus infection. Journal of Veterinary Internal Medicine, 32(2), 707-711. http://dx.doi.org/10.1111/jvim.15072. PMid:29460302.

Perley, K., Burns, C. C., Maguire, C., Shen, V., Joffe, E. R., Stefanovski, D., Redding, L., Germanis, L., Drobatz, K. J., & Watson, B. (2020). Retrospective evaluation of outpatient canine parvovirus treatment in a shelter-based low-cost urban clinic. Journal of Veterinary Emergency and Critical Care, 30(2), 202-208. http://dx.doi.org/10.1111/vec.12941. PMid:32096333.

Pollock, R. V. H., & Carmichael, L. E. (1983). Canine viral enteritis. The Veterinary Clinics of North America. Small Animal Practice, 13(3), 551-566. http://dx.doi.org/10.1016/S0195-5616(83)50059-4. PMid:6316616.

Quinn, P. J., Markey, B. K., Carter, M. E., Donnelly, W. J., & Leonard, F. C. (2005). Microbiologia veterinária e doenças infecciosas (1ª ed.). Porto Alegre: Artmed.

Reid, J., Nolan, A. M., Hughes, J. M. L., Lascelles, D., Pawson, P., & Scott, E. M. (2007). Development of the short-form Glasgow Composite Measure Pain Scale (CMPS-SF) and derivation of an analgesic intervention score. Animal Welfare, 16(Suppl. 1), 97-104. http://dx.doi.org/10.1017/S096272860003178X.

Rodrigues, B., Letícia, B., & Molinari, D. (2017). Diagnóstico e tratamento de parvovirose canina: Revisão de literatura. Brazilian Journal of Surgery and Clinical Research, 21(2), 127-134.

Sagai, M., & Bocci, V. (2011). Mechanisms of action involved in ozone therapy: Is healing induced via a mild oxidative stress? Medical Gas Research, 1(1), 29. http://dx.doi.org/10.1186/2045-9912-1-29. PMid:22185664.

Sciorsci, R. L., Lillo, E., Occhiogrosso, L., & Rizzo, A. (2020). Ozone therapy in veterinary medicine: A review. Research in Veterinary Science, 130, 240-246. http://dx.doi.org/10.1016/j.rvsc.2020.03.026. PMid:32234614.

Sechi, L. A., Lezcano, I., Nunez, N., Espim, M., Duprè, I., Pinna, A., Molicotti, P., Fadda, G., & Zanetti, S. (2001). Antibacterial activity of ozonized sunflower oil (Oleozon). Journal of Applied Microbiology, 90(2), 279-284. http://dx.doi.org/10.1046/j.1365-2672.2001.01235.x. PMid:11168731.

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Copyright (c) 2023 Tiago Gonçalves dos Santos, Jéssica Rodrigues Orlandin, Matheus Ferreira de Almeida, Rodrigo Ferreira Scassiotti, Vanessa Cristina Oliveira, Sarah Ingrid Pinto Santos, Vitória Mattos Pereira; Priscilla Avelino Ferreira Pinto; Clésio Gomes Mariano Junior, Carlos Eduardo Ambrósio