Insecticidal activity in vitro of the essential oil of Pogostemon cablin against Ctenocephalides felis felis


flea, volatile oil, sesquiterpene alcohol, patchouli.

How to Cite

Temperini, M. B. da S., Fortunato, A. B. R., Campos, D. R., de Jesus, I. L. R., Cid, Y. P., Scott, F. B., & Coumendouros, K. (2022). Insecticidal activity in vitro of the essential oil of Pogostemon cablin against Ctenocephalides felis felis. Brazilian Journal of Veterinary Medicine, 44, e003422.


The aim of this study was to evaluate the in vitro insecticidal activity of the essential oil (EO) of P. cablin against immature and adult stages of C. felis. The chemical composition of P. cablin EO was determined by gas chromatography (GC). To evaluate the insecticidal activity, the egg, larva, pupa, and adult stages of C. felis were exposed to different concentrations of P. cablin EO diluted in acetone and impregnated in filter paper in a concentration range of 25 to 200 μ After exposure, the material was incubated in climatized chambers and mortality assessment was performed after 24 h for the larval and adult stages, 72 h for the egg stage and 15 days for the pupal stage. After GC analysis, the major constituents of the EO of P. cablin were found to be patchoulol (27.4%), α-bulnesene (19.7%) and α-guayene (15.0%). The of mortality percentage was 100% at the concentration of 200 μ for all stages. The LCs50 values were 46.1, 49.2, 60.6 and 62.7 μ, respectively for the pupal, egg, larva and adult stages. Based on the results obtained, we can conclude that the EO of P. cablin contained patchoulol, α-bulnesene, and α-guayene as major constituents and had insecticidal activity against the immature and adult stages of C. felis.


Abbott, W. S. (1925). A method of computing the effectiveness of an insecticide. Journal of Economic Entomology, 18(2), 265-267.

Adams, R. P. (2007). Identification of essential oil components by gas chromatography/mass spectrometry (Vol. 456, pp. 544-545). Allured Publishing Corporation.

Conceição, C. L., de Morais, L. A. S., Campos, D. R., Chaves, J. K. O., dos Santos, G. C. M., Cid, Y. P., de Sousa, M. A. A., Scott, F. B., Chaves, D. S. A., & Coumendouros, K. (2020). Evaluation of insecticidal activity of thyme, oregano, and cassia volatile oils on cat flea. Revista Brasileira de Farmacognosia, 30(6), 774-779. http://dx.doi. org/10.1007/s43450-020-00111-8.

Costa, G. A., Carvalho Filho, J. L. S., & Deschamps, C. (2013). Rendimento e composição do óleo essencial de patchouli (Pogostemon cablin) conforme o tempo de extração. Revista Brasileira de Plantas Medicinais, 15(3), 319-324.

Freitas, J. P., de Jesus, I. L. R., Chaves, J. K. O., Gijsen, I. S., Campos, D. R., Baptista, D. P., Ferreira, T. P., Alves, M. C. C., Coumendouros, K., Cid, Y. P., & Chaves, D. S. A.. (2021). Efficacy and residual effect of Illicium verum (star anise) and Pelargonium graveolens (rose geranium) essential oil on cat fleas Ctenocephalides felis felis. Revista Brasileira de Parasitologia Veterinária, 30(4), e009321. PMid:34910016.

Lappin, M. R., Tasker, S., & Roura, X. (2020). Role of vector-borne pathogens in the development of fever in cats: 1. Flea-associated diseases. Journal of Feline Medicine and Surgery, 22(1), 31-39. http://dx.doi. org/10.1177/1098612X19895941. PMid:31916873.

Lima Santos, L., Barreto Brandão, L., Lopes Martins, R., de Menezes Rabelo, E., Lobato Rodrigues, A. B., da Conceição Vieira Araújo, C. M., Fernandes Sobral, T., Ribeiro Galardo, A. K., & Moreira da Silva de Ameida, S. S. (2019). Evaluation of the larvicidal potential of the essential oil Pogostemon cablin (Blanco) Benth in the control of Aedes aegypti. Pharmaceuticals, 12(2), 53. PMid:30965561.

Pavela, R., & Benelli, G. (2016). Essential oils as ecofriendly biopesticides? Challenges and constraints. Trends in Plant Science, 21(12), 1000-1007. PMid:27789158.

RStudio. (2020). RStudio: Integrated Development Environment for R. RStudio, PBC.

Rust, M. K. (2020). Recent advancements in the control of cat fleas. Insects, 11(10), 668. PMid:33003488.

Santos, A. A., Farder-Gomes, C. F., Ribeiro, A. V., Costa, T. L., França, J. C. O., Bacci, L., Demuner, A. J., Serrão, J. E., & Picanço, M. C.. (2022). Lethal and sublethal effects of an emulsion based on Pogostemon cablin (Lamiaceae) essential oil on the coffee berry borer, Hypothenemus hampei. Environmental Science and Pollution Research International, 29(30), 45763. PMid:35152351.

Creative Commons License

This work is licensed under a Creative Commons Attribution 4.0 International License.

Copyright (c) 2022 Micaella Bastos da Silva Temperini, Anna Beatriz Ribeiro Fortunato, Diefrey Ribeiro Campos, Ingrid Lins Raquel de Jesus, Yara Peluso Cid, Fabio Barbour Scott, Katherina Coumendouros