Keywords
How to Cite
Abstract
The fungi Arthrobotrys musiformis (A144 isolate) and Monacrosporium sinense (SF53 isolate) were evaluated for controlling L3 of cyathostomins. In vitro, Petri dishes containing 2% water-agar were divided into groups: 1 (A144 + SF53); 2 (SF53); 3 (A144); 4 (control group). In vivo, 24 animals received pellets containing fungal mycelium. The animals were divided into four groups: 1 (100 g of pellets containing A144 + SF53 isolates); 2 (100 g of pellets containing A144 isolate); 3 (100 g of pellets containing SF53 isolate); 4 (Pellets without fungi). Feces were collected at 12, 24, 36, 48, 60 and 72 hours after pellet administration. The association of isolates, in vitro, was able to reduce L3 by 69.7%, compared with the control group; A144 isolate reduced L3 by 77.3%; and SF53 isolate reduced L3 by 84.3%. In vivo, the association of fungi A. musiformis and M. sinense showed predatory activity against L3 of cyathostomins with efficacy ranged from 63.2% to 83%. The association of the fungi A. musiformis and M. sinense both in vitro and in vivo proved to be efficient for controlling L3 of cyathostomins.
References
Araujo, J.M., Araújo, J.V., Braga, F.R., Carvalho, R.O. (2010). In vitro predatory activity of nematophagous fungi and after passing through gastrointestinal tract of equine on infective larvae of Strongyloides westeri. Parasitology Research, 107,103-107. https://doi.org/10.1007/s00436-010-1841-y.
Assis, R. C. L., Luns, F. D., Araújo, J. V., & Braga, F. R. (2012). Biological control of trichostrongyles in beef cattle by the nematophagous fungus Duddingtonia flagrans in tropical southeastern Brazil. Experimental Parasitology, 132(3), 373-377. http://dx.doi.org/10.1016/j.exppara.2012.08.013. PMid:22975475.
Ayres, M., Ayres, J. R. M., Ayres, D. L., & Santos, A. S. (2007). Aplicações estatísticas nas áreas de ciências biológicas. Brasília: CNPq.
Ayupe, T. H., Monteiro, T. S. A., Braga, F. R., Soares, F. E. F., Mello, I. K., & Araújo, J. V. (2016). Assessment of compatibility between the nematophagous fungi Arthrobotrys robusta and Duddingtonia flagrans under laboratory conditions. Revista Iberoamericana de Micologia, 33(2), 129-130. http://dx.doi.org/10.1016/j. riam.2015.07.001. PMid:26631950.
Braga, F. R., Araújo, J. V., Carvalho, R. O., Araújo, J. M., Silva, A. R., & Campos, A. K. (2009). Controle in vitro de larvas infectantes de ciatostomíneos (NEMATODA: CYATHOSTOMINAE) de equinos utilizando os fungos predadores Duddingtonia flagrans, Monacrosporium thaumasium e Arthrobotrys robusta. Ciência Animal Brasileira, 10(3), 887-892. https://www.revistas.ufg.br/vet/article/view/2720
Castro, A. A., Oliveira, C. R. C., Anjos, D. H. S., De Ornelas, E. I., Bittencourt, V. R. E. P., Araújo, J. V., Sampaio, I. B. M., & Rodrigues, M. L. A. (2003). Potencial dos fungos nematófagos Arthrobotrys sp. e Monacrosporium thaumasium para o controle de larvas de ciatostomíneos de equinos (Nematoda: Cyathostominae). Revista Brasileira de Parasitologia Veterinária, 12(2), 53-57.
Costa, P. W. L., Alvares, F. B. V., Bezerra, R. A., Sarmento, W. F., Silva, F. F., Rodrigues, J. A., Ferreira Feitosa, T., Araújo, J. V., Braga, F. R., & Vilela, V. L. R. (2019). Effect of refrigeration storage of nematophagous fungi embedded in sodium alginate pellets on predatory activity against asinine gastrointestinal nematodes. Biocontrol Science and Technology, 19(11), 1106-1117. http://dx.doi.org/10.1080/09583157.2019.1658180.
Dalio, R.J., Fleischmann, F., Humez, M., Osswald, W. (2014). Phosphite protects Fagus sylvatica seedlings towards Phytophthora plurivora via local toxicity, priming and facilitation of pathogen recognition. Plos One, 9(1): e87860. https://doi.org/10.1371/journal.pone.0087860.
Dalla Pria, M., & Ferraz, S. (1996). Controle biológico de Meloidogyne incognita, raça 3, por seis espécies de Monacrosporium, isoladas ou combinadas com Verticillium chlamydosporium. Fitopatologia Brasileira, 21, 30-34.
Ferraz, C. M., Silva, L. P. C., Soares, F. E. F., Souza, R. L. O., Tobias, F. L., Araújo, J. V., Veloso, F. B. R., Laviola, F. P., Endringer, D. C., de Gives, P. M., & Braga, F. R. (2020). Effect of silver nanoparticles (AgNP’s) from Duddingtonia flagrans on cyathostomins larvae (subfamily: Cyathostominae). Journal of Invertebrate Pathology, 174, 107395. http://dx.doi.org/10.1016/j.jip.2020.107395. PMid:32433916.
Godéski, A., Pedrassani, D., Szczerbowski, C.R. (2017). Eficácia de anti-helmínticos em equinos da raça Crioula no município de Major Vieira/SC. Revista Acadêmica Ciência Animal, 15, 59-66. https://doi.org/10.7213/ academica.15.2017.08.
Instituto Brasileiro de Geografia e Estatística – IBGE. (2018). Produção da pecuária municipal. https://biblioteca. ibge.gov.br/visualizacao/periodicos/84/ppm_2018_v46_br_informativo.pdf
Kaplan, R. M., & Nielsen, M. K. (2010). An evidence-based approach to equine parasite control: It ain’t the 60s anymore. Equine Veterinary Education, 22(6), 306-316. http://dx.doi.org/10.1111/j.2042-3292.2010.00084.x.
Lackey, B. A., Muldoon, A. E., & Jaffee, B. A. (1993). Alginate pellet formulation of Hirsutella rossiliensis for biological control of plant-parasitic nematodes. Biological Control, 3(2), 155-160. http://dx.doi.org/10.1006/bcon.1993.1023.
Madeira de Carvalho, L. M., Fazendeiro, M. I., & Afonso-Roque, M. M. (2004). Estudo morfométrico das larvas infectantes (L3) dos estrongilídeos (Nematoda: Strongylidae) dos equídeos - 1. Género Cyathostomum s.l. Acta Parasitológica Portuguesa, 11(1-2), 23-32.
Madeira de Carvalho, L. M., Fazendeiro, M. I., & Afonso-Roque, M. M. (2008). Estudo morfométrico das larvas infectantes (L3) dos estrongilídeos (Nematoda: Strongylidae) dos equídeos. 4. Estudo das populações de equídeos bravios e domésticos através do método de análise dos morfotipos de L3 de Cyathostomum sensu latum. Acta Parasitológica Portuguesa, 15(1-2), 65-70.
Nielsen, M. K., Mittel, L., Grice, A., Erskine, M., Graves, E., Vaala, W., Tully, R. C., French, D. D., Bowman, R., & Kaplan, R. M. (2019). AAEP parasite control guidelines, american association of equine practitioner. https://aaep.org/ sites/default/files/Guidelines/AAEPParasiteControlGuidelines_0.pdf
Paz-Silva, A., Francisco, I., Valero-Coss, R. O., Cortinasa, F. J., Sánchez, J. A., Francisco, R., Arias, M., Suárez, J. L., López-Arellano, M. E., Sánchez-Andrade, R., & Mendoza de Gives, P. (2011). Ability of the fungus Duddingtonia flagrans to adapt to the cyathostomin egg-output by spreading chlamydospores. Veterinary Parasitology, 79(1-3), 277-282. http://dx.doi.org/10.1016/j.vetpar.2011.02.014. PMid:21402449.
Peregrine, A., Molento, M. B., Kaplan, R. M., & Nielsen, M. K. (2014). Anthelmintic resistance in important parasites of horses: Does it really matter? Veterinary Parasitology, 201(1-2), 1-8. http://dx.doi.org/10.1016/j. vetpar.2014.01.004. PMid:24485565.
Piccoli, C., Marques, S. M. T., Appel, G., Da Silveira, E., Siqueira, G. B., Loos, D. E., & Mattos, M. J. T. (2015). Helmintos intestinais em cavalos de trabalho e de lazer de Porto Alegre/RS. Science and Animal Health, 3(1), 56-64. http:// dx.doi.org/10.15210/sah.v3i1.4227.
Rodrigues, J. Á., Alvares, F. B. V., Silva, J. T., Ferreira, L. C., Lopes da Costa, P. W., Sarmento, W. F., Ferreira Feitosa, T., Araújo, J. V., Braga, F. R., & Longo Ribeiro Vilela, V. (2020). Predatory effects of the fungus Arthrobotrys cladodes on sheep gastrointestinal nematodes. Biological Science and Technology, 30(8), 830-839. http://dx.doi. org/10.1080/09583157.2020.1775176.
Tavela, A. O., Araújo, J. V., Braga, F. R., Araujo, J. M., Magalhães, L. Q., Silveira, W. F., & Borges, L. A. (2012). In vitro association of nematophagous fungi Duddingtonia flagrans (AC001), Monacrosporium thaumasium (NF34) and Pochonia chlamydosporia (VC1) to control horse cyathostomin (Nematoda: Strongylidae). Biocontrol Science and Technology, 22(5), 607-610. http://dx.doi.org/10.1080/09583157.2012.672952.
Tavela, A. O., Araújo, J. V., Braga, F. R., Silveira, W. F., Dornelas e Silva, V. H., Carretta Júnior, M., Borges, L. A., Araujo, J. M., Benjamin, L. A., Carvalho, G. R., & Paula, A. T. (2013). Coadministration of sodium alginate pellets containing the fungi Duddingtonia flagrans and Monacrosporium thaumasium on cyathostomin infective larvae after passing through the gastrointestinal tract of horses. Research in Veterinary Science, 94(3), 568- 572. http://dx.doi.org/10.1016/j.rvsc.2012.11.011. PMid:23274060.
Ueno, H., & Gonçalves, P. C. (1998). Manual para diagnóstico das helmintoses de ruminantes (4. ed.). Tokyo: JICA.
Walker, H. L., & Connick Junior, W. J. (1983). Sodium alginate for production and formulation of mycoherbicides. Weed Science, 31(3), 333-338. http://dx.doi.org/10.1017/S0043174500069113.
This work is licensed under a Creative Commons Attribution 4.0 International License.
Copyright (c) 2021 Atílio Dalcin Júnior, Vinicius Monteiro Ferreira, Lorendane Millena de Carvalho, Felipe Boniedj Ventura Álvares, Vinicius Longo Ribeiro Vilela, Carolina Magri Ferraz, Francielle Bosi Rodrigues Veloso, Thalita Fonseca Lima, Fabio Ribeiro Braga, Jackson Victor de Araújo